We narrowed to 8,212 results for: Dos

1. pCUU

   Plasmid

   #159747

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-MKP7_K62R_153

   Plasmid

   #37795

   Depositor

   SeWon Suh

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

   Insert

   Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Lysnie 62 to Arginine and deleted amino a…

   Promoter

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET21a-MKP7_K52R_153

   Plasmid

   #37793

   Depositor

   SeWon Suh

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

   Insert

   Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Lysnie 52 to Arginine and deleted amino a…

   Promoter

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET21a-MKP7_wild_153

   Plasmid

   #37792

   Depositor

   SeWon Suh

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

   Insert

   Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 154-665

   Promoter

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pPT24

   Plasmid

   #184419

   Purpose

   Transformation of mitochondrial DNA in yeast

   Depositor

   Martin Ott

   Article

   Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA310

   Plasmid

   #190002

   Purpose

   Inducible expression vector for Trypanosoma brucei

   Depositor

   Bungo Akiyoshi

   Article

   Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

   Type

   Empty backbone

   Use

   Expression vector for trypanosoma brucei

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti-sgRNA puro

   Plasmid

   #104990

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lenti-sgRNA neo

   Plasmid

   #104992

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcw57.1-RquA-FLAG

    Plasmid

    #229685

    Purpose

    Doxycycline-inducible expression of RquA-FLAG

    Depositor

    Jessica Spinelli

    Article

    Valeros et al Cell. 2025 Jan 10:S0092-8674(24)01420-X.

    Insert

    RquA-FLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pF CAG luc-EGFP-cre puro

    Plasmid

    #67503

    Purpose

    Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    luciferase-EGFP-cre
    

    Use

    Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYEE

    Plasmid

    #159750

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-N2H-N1

    Plasmid

    #125547

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to n-terminus of Gatew…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-N2H-C1

    Plasmid

    #125549

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-N2H-C2

    Plasmid

    #125559

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-N2H-N2

    Plasmid

    #125548

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to n-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBMN DHFR-HiBiT / Lyt2

    Plasmid

    #179731

    Purpose

    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

    Depositor

    James Inglese

    Article

    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

    Insert

    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Human, Mouse)
    

    Use

    Retroviral

    Tags

    HiBiT

    Expression

    Mutation

    Promoter

    LTR/MMLV

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTEE

    Plasmid

    #159748

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-N2H-F2-empty vector

    Plasmid

    #125552

    Purpose

    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge