We narrowed to 987 results for: Streptococcus

1. pCY5.1kn

   Plasmid

   #160298

   Purpose

   Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJCC_164 SpCas9 R1333A/R1335A

   Plasmid

   #179528

   Purpose

   For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 R1333A/R1335A
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginines 1333 and 1335 to alanine

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_103 SpCas9 T1337C

   Plasmid

   #179525

   Purpose

   For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 T1337C
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed threonine 1337 to cysteine

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-TEV-GB1 Initial Gly

   Plasmid

   #128685

   Purpose

   Expresses GB1 mutant with no Methionine residues

   Depositor

   Sharon Rozovsky

   Article

   Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

   Insert

   spg
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. His-TEV-GB1 Ile6Met

   Plasmid

   #128687

   Purpose

   Expresses GB1 Ile6Met mutant

   Depositor

   Sharon Rozovsky

   Article

   Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

   Insert

   spg
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. His-TEV-GB1 Val29Met

   Plasmid

   #128688

   Purpose

   Expresses GB1 Val29Met mutant

   Depositor

   Sharon Rozovsky

   Article

   Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

   Insert

   spg
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. His-TEV-GB1 Ala34Met

   Plasmid

   #128689

   Purpose

   Expresses GB1 Ala34Met mutant

   Depositor

   Sharon Rozovsky

   Article

   Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

   Insert

   spg
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. His-TEV-GB1 Val39Met

   Plasmid

   #128690

   Purpose

   Expresses GB1 Val39Met mutant

   Depositor

   Sharon Rozovsky

   Article

   Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

   Insert

   spg
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. His-TEV-GB1 Val54Met

    Plasmid

    #128691

    Purpose

    Expresses GB1 Val54Met mutant

    Depositor

    Sharon Rozovsky

    Article

    Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

    Insert

    spg
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT140_GalL_cCDA1-VRERBE3

    Plasmid

    #145103

    Purpose

    Expressing base editor cCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VRERBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT46_GalL_cCDA1-BE3

    Plasmid

    #145039

    Purpose

    Expresses cCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    cCDA1-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT176_GalL_eA3A-BE3

    Plasmid

    #145125

    Purpose

    Expressing base editor eA3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(N57G)-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(N57G); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT171_GalL_A3AΔ178-BE3

    Plasmid

    #145121

    Purpose

    Expressing base editor A3AΔ178-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ178-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(1-178AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT170_GalL_A3AΔ182-BE3

    Plasmid

    #145120

    Purpose

    Expressing base editor A3AΔ182-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ182-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(1-182AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT169_GalL_A3AΔ186-BE3

    Plasmid

    #145119

    Purpose

    Expressing base editor A3AΔ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ186-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT166_GalL_A3A-BE3

    Plasmid

    #145116

    Purpose

    Expressing base editor A3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT139_GalL_nCDA1-VRERBE3

    Plasmid

    #145102

    Purpose

    Expressing base editor nCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VRERBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT122_GalL_cCDA1-VQRBE3

    Plasmid

    #145087

    Purpose

    Expressing base editor cCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VQRBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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