We narrowed to 9,384 results for: Coli

1. pET His10-Draper INT

   Plasmid

   #126670

   Purpose

   Expression of His10-Draper intracellular domain for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic domain
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234453

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234454

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET32bplus_trx_dAChE4

   Plasmid

   #83917

   Purpose

   Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

   Depositor

   Sarel Fleishman

   Article

   Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

   Insert

   Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
   

   Use

   Tags

   His, S-tag, and TRX

   Expression

   Bacterial

   Mutation

   V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…

   Promoter

   T7 promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

   Plasmid

   #169191

   Purpose

   To express nsp10-nsp16 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

   Insert

   14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLXI_TRC403 LacZ

   Plasmid

   #111184

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   TRE

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLXI_TRC401 LacZ

   Plasmid

   #111183

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   TRE

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

   Plasmid

   #209083

   Purpose

   SNCA (Avi tagged) expression along with BirA ligase

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   BirA
   

   Use

   Tags

   Avi

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

   Plasmid

   #209084

   Purpose

   Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   NGN2 (Neurog2 Mouse)
   BirA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   hSyn

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_BRD003 Luciferase

    Plasmid

    #117072

    Purpose

    single guide RNA targeting Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HemH-R212A_pET-14b

    Plasmid

    #233262

    Purpose

    Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

    Depositor

    Mohan Babu, Andrew Emili

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    HemH (hemH )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 21…

    Promoter

    T7 promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Use

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG15-pBAD-tetO-deGFP

    Plasmid

    #102450

    Purpose

    Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD-tetO

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIMAY

    Plasmid

    #68939

    Purpose

    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

    Depositor

    Tim Foster

    Article

    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

    Type

    Empty backbone

    Use

    Staphylococcal allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Tags

    SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Stephen Quake, Paul Blainey

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIII/MS2-1

    Plasmid

    #220629

    Purpose

    Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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