We narrowed to 10,047 results for: yeast

1. pNC1124

   Plasmid

   #41560

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9
   

   Use

   Tags

   Expression

   Mutation

   GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

   Promoter

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Use

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBJ1806 pRS413-COF1RFP

   Plasmid

   #37101

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Promoter

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDS24

   Plasmid

   #58952

   Purpose

   Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

   Depositor

   Thomas Fox

   Article

   Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

   Insert

   Mitochondrially coded Arg8 protein
   

   Use

   Tags

   Expression

   Mutation

   Coding sequence synthetic in S. cerevisiae mitoch…

   Promoter

   n/a

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-EGFP-C1-HsNXF-1_K

   Plasmid

   #146740

   Purpose

   Mammalian Expression of HsNxf-1

   Depositor

   Elisa Izaurralde

   Article

   Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

   Insert

   HsNxf-1 (NXF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S144G relative to NM_006362.4

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUMO-YHRC

   Plasmid

   #54336

   Purpose

   Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

   Depositor

   Claes Andréasson

   Article

   Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

   Type

   Empty backbone

   Use

   Tags

   6His-SUMO (yeast gene: SMT3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7-LacO

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS406 ATG9 stopGFP

   Plasmid

   #114669

   Purpose

   Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Changed the three linker base pairs after the las…

   Promoter

   ATG9

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCTcon2-5INS_16

    Plasmid

    #102292

    Purpose

    Yeast Surface Display of 5Ins_16 scFV

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS426-GFP-Osh2-PH

    Plasmid

    #71373

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCTcon2-5INS_EV3

    Plasmid

    #102293

    Purpose

    Yeast Surface Display of 5Ins_16_EV3

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Evolved Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS406-PHO5-GFP-OSBP PH domain

    Plasmid

    #58840

    Purpose

    Expresses GFP-Tagged OSBP PH domain in yeast

    Depositor

    Sean Munro, Tim Levine

    Article

    Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

    Insert

    Oxysterol-binding Protein PH domain (OSBP Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRS426-GFP-Osh1-PH

    Plasmid

    #71372

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh1-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa267-388

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pG23A

    Plasmid

    #102884

    Purpose

    Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHO_pTEFmut7_CggR_R250A_ble

    Plasmid

    #124585

    Purpose

    Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    cggR_R250A (cggR Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    changed arginine 250 to alanine

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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