19,654 results

1. pLV-Ftractin-mCherry

   Plasmid

   #85131

   Purpose

   lentiviral expression of an F-actin live-cell marker (mCherry)

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223 SARS-CoV-2 N

   Plasmid

   #149330

   Purpose

   Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   N (N SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1_PA-Cre

   Plasmid

   #122960

   Purpose

   Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_PA-Cre
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET N6his GFP1-9

   Plasmid

   #182240

   Purpose

   Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Insert

   GFP1-9
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-NL1(-)

   Plasmid

   #15260

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Neuroligin 1 (Nlgn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-EGFP

   Plasmid

   #21181

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   enhanced green flourescent protein
   

   Use

   Drosophila expression

   Available Since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

   Plasmid

   #110057

   Purpose

   Per2 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Per2 promoter and Venus (Per2 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   NLS-D2

   Expression

   Mammalian

   Promoter

   Per2

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-NRP1

   Plasmid

   #158384

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   NRP1 (NRP1 Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-GFP-IMP2-2

   Plasmid

   #42175

   Depositor

   Alexandra Kiemer

   Article

   Kessler et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-FLAG-Luciferase

    Plasmid

    #58792

    Purpose

    Express FLAG-tagged Luciferase in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    firefly Luciferase
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5FRT/Flag-WDR5

    Plasmid

    #29759

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBa.TfR.GFP

    Plasmid

    #45060

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Burack et al Neuron. 2000 May;26(2):465-72.

    Insert

    TfR (TFRC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Chicken Beta Actin

    Available Since

    Oct. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. hsgk1

    Plasmid

    #83432

    Purpose

    full length human sgk1 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    human Sgk1 (SGK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. G1A

    Plasmid

    #24690

    Depositor

    Jeanne Lawrence

    Article

    Clemson et al J Cell Biol. 1996 Feb . 132(3):259-75.

    Insert

    human XIST (XIST Human)
    

    Use

    Plasmid cloning vector

    Mutation

    Genomic insert. Includes introns 5-8.

    Available Since

    Jan. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLH02

    Plasmid

    #117262

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-LVISKB_1732-30 , sapK-sappR
    

    Use

    Other

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNatA (pACYCduet-naa10-naa15)

    Plasmid

    #72928

    Purpose

    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    naa10 (naa10 Fission Yeast, Synthetic)
    naa15 (naa15 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Codon optomised for E.coli expression

    Promoter

    T7

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM-CBE

    Plasmid

    #196293

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding CBE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN3-MGA

    Plasmid

    #107715

    Purpose

    Expression plasmid for 3xFLAG-tagged MGA

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Insert

    MGA (MGA Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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