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pSUMO3

Plasmid

#52260

Purpose

Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-DDB1

Plasmid

#19918

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-TRPM1 cDNA

Plasmid

#29782

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

TRPM1 (TRPM1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLUT7 HA-GLI1

Plasmid

#62970

Purpose

Expresses HA-tagged Gli1

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBMPC3

Plasmid

#31367

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Bmal1 (Bmal1 Mouse)

Tags

His

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-mito-DAAO

Plasmid

#141132

Purpose

Expresses DAAO with mitochondrial localization signal in mammalian cells

Depositor

Vsevolod Belousov

Insert

mito-DAAO

Tags

2 mitochondrial localization signals and 7xHis

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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ePop

Plasmid

#50953

Purpose

Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

Depositor

Lingchong You

Article

Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

Inserts

E lysis protein
LuxI
LuxR

Mutation

Early stop codon introduced in mutated luxR resul…

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-FAM134B

Plasmid

#128260

Purpose

Expresses FAM134B in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134B (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19 Giles integrase

Plasmid

#170049

Purpose

pUC19 with Giles integrase

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Insert

GIles Integrase

Expression

Bacterial

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Plasmid

#175777

Purpose

pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TMEM192 (TMEM192 Human)

Use

CRISPR

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA2h

Plasmid

#140554

Purpose

Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2h (high affinity)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-ACEmax

Plasmid

#139105

Purpose

Mammalian Target-ACEmax expression vector (pSI-625)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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