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20,359 results

Showing: 5641 - 5660 of 20359 results

  6. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Publication
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133451-plasmid-map-sequence-id-267048

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  10. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107943-plasmid-map-sequence-id-207614

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  14. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    Zhe J. Liu
    Publication
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91564-plasmid-map-sequence-id-180643

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  18. pcDNA3.1-ccdB-Nanoluc

    Plasmid
    #87067
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Available since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87067-plasmid-map-sequence-id-192710

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  19. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Expression
    Yeast
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83476-plasmid-map-sequence-id-189195

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Showing: 5641 - 5660 of 20359 results