59,541 results

1. pLVX-HA-UBE3B-IRES-Puro

   Plasmid

   #107345

   Purpose

   Expresses HA tagged UBE3B in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. p3258 pCMV hUBC9 wt HA

   Plasmid

   #14438

   Depositor

   Peter Howley

   Article

   Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. ALPK1 gRNA (BRDN0001146195)

   Plasmid

   #76082

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-miniTurbo-V5-CDK20_IDG-K

   Plasmid

   #189883

   Purpose

   Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK20 (CDK20 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ALPK1 gRNA (BRDN0001146035)

   Plasmid

   #76081

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALPK1 gRNA (BRDN0001145294)

   Plasmid

   #76083

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. ALPK1 gRNA (BRDN0001146875)

   Plasmid

   #76084

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FCGR2a-bio-His

   Plasmid

   #51754

   Purpose

   Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FCGR2a (FCGR2A Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. TARDBP

    Plasmid

    #156073

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX_hDDX5_FL_wt

    Plasmid

    #88871

    Purpose

    inducible expression of GST-DDX5 in bacteria

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0275

    Plasmid

    #142582

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T-1-6AlanineHE1

    Plasmid

    #165098

    Purpose

    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1

    Depositor

    Arthur Haas

    Insert

    Uba1 (UBA1 Human)
    

    Tags

    GST-6-alanine

    Expression

    Bacterial

    Mutation

    None

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.3 LRP5

    Plasmid

    #115907

    Purpose

    Expresses human LRP5 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. STING-V2

    Plasmid

    #124264

    Purpose

    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Tags

    Venus2

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AnkyrinB-2XHA

    Plasmid

    #31057

    Depositor

    Vann Bennett

    Article

    Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

    Insert

    Ankyrin B (ANK2 Human)
    

    Tags

    2XHA

    Expression

    Mammalian

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only