We narrowed to 11,691 results for: NSI

1. pHH0103_VAV1-1/2

   Plasmid

   #91355

   Purpose

   Protein expression and purification of human SH3 domain construct VAV1-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   VAV1-1/2 (VAV1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM549

   Plasmid

   #112531

   Purpose

   pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   

   Expression

   Bacterial

   Promoter

   J23107 and ProD

   Available Since

   Feb. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BAS290 C terminal E2C natR

   Plasmid

   #74085

   Purpose

   S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   E2Crimson
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pHH0103_STAM-1/1

   Plasmid

   #91428

   Purpose

   Protein expression and purification of human SH3 domain construct STAM-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   STAM-1/1 (STAM Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac Dual PNG-FLAG PLU-HIS

   Plasmid

   #113173

   Purpose

   Baculovirus expression of PNG and PLU

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   PNG, PLU
   

   Use

   Baculovirus

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad-CA TAG233

   Plasmid

   #105838

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 233

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. sh-Myocardin

   Plasmid

   #100768

   Purpose

   expression of shRNA targeting MYOCD

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   sh-Myocardin
   

   Use

   RNAi

   Promoter

   H1

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_PACSIN3-1/1

   Plasmid

   #91335

   Purpose

   Protein expression and purification of human SH3 domain construct PACSIN3-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PACSIN3-1/1 (PACSIN3 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-RsACR_995-EYFP

   Plasmid

   #103772

   Purpose

   Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin RsACR_995
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GcACR_457-EYFP

    Plasmid

    #103137

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_457
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. M26V DJ-1 pET15b

    Plasmid

    #60688

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26V DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26V

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pKDsgRNA-trmI

    Plasmid

    #89955

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    trmI gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJW3.42

    Plasmid

    #62866

    Purpose

    Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    PPTR-1/pLexA-N bait
    

    Use

    Two-hybrid bait vector

    Tags

    LexA

    Expression

    Yeast

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTAtUbq3 (GB0219)

    Plasmid

    #68192

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq3 terminator
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB085d7

    Plasmid

    #79211

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB085d6

    Plasmid

    #79210

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PFA0445w-bio-His

    Plasmid

    #50811

    Purpose

    Expresses enzymatically monobiotinylated full length PFA0445w

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PFA0445w
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PF10_0166-bio-His

    Plasmid

    #50801

    Purpose

    Expresses enzymatically monobiotinylated full length PF10_0166

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PF10_0166
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-B3

    Plasmid

    #31707

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-20b (Mir20b Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-R1ACR_741-EYFP

    Plasmid

    #103961

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R1ACR_741
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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