We narrowed to 9,109 results for: control

1. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

   Plasmid

   #208174

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
   

   Use

   Tags

   Strep-tag

   Expression

   Mammalian

   Mutation

   Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JO-pH720-SARS-CoV-2-S-tail-WT

   Plasmid

   #218453

   Purpose

   Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
   

   Use

   Tags

   eXact

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

   Plasmid

   #218463

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lys1269Ala and His1271Ala substitutions

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218915

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218913

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 5335T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218912

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 5335T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

   Plasmid

   #210709

   Purpose

   This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-hU6-shBACH1_BFP

   Plasmid

   #199217

   Purpose

   Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   shBACH1 (BACH1 Synthetic)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC_hU6-shRKIP_BFP

   Plasmid

   #199216

   Purpose

   Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   shBACH1 (BACH1 Synthetic)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUt-mNF-GFP

    Plasmid

    #199220

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-R/G-ICUE

    Plasmid

    #181849

    Purpose

    ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    R/G-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. I282L

    Plasmid

    #184308

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I282L

    Promoter

    AOX1

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. I175L

    Plasmid

    #184322

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I175L

    Promoter

    AOX1

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSmEn2ERT(#117)

    Plasmid

    #184079

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSmEn2n4ERT(#234)

    Plasmid

    #184082

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(n4)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSmEn2SRERT(#231)

    Plasmid

    #184080

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(SR)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

    Promoter

    sCMV+SP6

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT22UbhaEng2aE4PCh(#271)

    Plasmid

    #184085

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22UbhaEng2bE4PCh(#272)

    Plasmid

    #184086

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT22gCfpUbm5En2E4PCh(#251)

    Plasmid

    #184083

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT22Ubm5Eng2bE4PCh(#284)

    Plasmid

    #184088

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge