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IDP425

Plasmid

#87022

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-1xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Ncam1_1.18.1-AP-His

Plasmid

#71962

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.18.1 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
MigR1 Fli-1 GFP

Plasmid

#24668

Depositor

Yaacov Ben-David

Article

Cui et al Blood. 2007 Oct 1. 110(7):2631-40.

Insert

Fli-1 (Fli1 Mouse)

Use

Retroviral

Tags

GFP and IRES

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
Flrt3-AP-His

Plasmid

#71949

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt3 (Flrt3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Neuropeptide Y [L115/13R-2b]

Plasmid

#206639

Purpose

Mammalian Expression Plasmid of anti-Neuropeptide Y (Human). Derived from hybridoma L115/13-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuropeptide Y (Homo sapiens) recombinant Mouse monoclonal antibody (NPY Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Shank [N23B/49R]

Plasmid

#128637

Purpose

Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Shank Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MyD88-NpLxIS

Plasmid

#131347

Purpose

Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-NpLxIS (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-NrCAM [N343/26R]

Plasmid

#177534

Purpose

Mammalian Expression Plasmid of anti-NrCAM (Mouse). Derived from hybridoma N343/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NrCAM (Mus musculus) recombinant mouse monoclonal antibody (Nrcam Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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mKO2-MyosinIIA-C-18

Plasmid

#57887

Purpose

Localization: Cytoskeleton, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Insert

MyosinIIA

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
Ncam1_0.0.0-AP-His

Plasmid

#71960

Purpose

Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_0.0.0 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Ascl1

Plasmid

#74338

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

achaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Promoter

-

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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GluN3A/NR3A glutamate receptor scFv [N416/57]

Plasmid

#206773

Purpose

Mammalian Expression of GluN3A/NR3A glutamate receptor scFV. Derived from hybridoma N416/57 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN3A/NR3A glutamate receptor (Mus musculus) recombinant scFV (Grin3a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Neuroligin-2 scFv [L107/90]

Plasmid

#206763

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/36]

Plasmid

#190563

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFV. Derived from hybridoma N59/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant scFV (Grin2b Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4

Plasmid

#136611

Purpose

Dox-inducible lentiviral vector expressing mouse Oct4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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