159,332 results

1. pFastBac-His-Tipin

   Plasmid

   #22891

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pDEST-V1-ORF

   Plasmid

   #73635

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 1 (V1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Vinculin-venus

   Plasmid

   #27300

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   Vinculin (VCL Chicken)
   

   Use

   Tags

   Venus A206K

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH-SFB

   Plasmid

   #99391

   Purpose

   Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrcHIS-wt7D12

   Plasmid

   #125268

   Purpose

   Expresses wild type 7D12 anti-EGFR nanobodies

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 nanobody
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNAGuide_003

   Plasmid

   #192505

   Purpose

   For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST-V2-ORF

   Plasmid

   #73636

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-LAMP1

   Plasmid

   #207787

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MBP-FUS_FL_WT

   Plasmid

   #98651

   Purpose

   Expresses MBP-tagged full length FUS

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLL3.7 EGFPC2 TAZ

    Plasmid

    #66850

    Purpose

    Express GFP-fused TAZ by lentivirus

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    WWTR1/TAZ (WWTR1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMMTV neu NT

    Plasmid

    #1823

    Depositor

    Philip Leder

    Article

    Muller et al Cell 1988 Jul 1;54(1):105-15.

    Insert

    neu NT (Erbb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

12. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Use

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-TRE3G-PYL1-sfGFP-PML

    Plasmid

    #167011

    Purpose

    expression of PYL1-SfGFP-PML, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    PML (PML Human)
    

    Use

    Lentiviral

    Tags

    GFP-PML

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE60-EFG-His

    Plasmid

    #124126

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor G (fusA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Pediculus humanus corporis PINK1 (115 - 575)

    Plasmid

    #110750

    Purpose

    Bacterial expression of PhPINK1 (biochemistry)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MaLionG

    Plasmid

    #113906

    Purpose

    Green ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CMV-puro_FlnaABD-TS-16flag

    Plasmid

    #234868

    Purpose

    Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid

    #234869

    Purpose

    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge