We narrowed to 11,977 results for: pcDNA

1. pcDNA-dCas9-p300 Core (D1399Y)

   Plasmid

   #61358

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

   Plasmid

   #204694

   Purpose

   This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   direct fusion of BRD4 and NUT

   Promoter

   CMV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-SARS-CoV-2-S-RBD-8his

   Plasmid

   #145145

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Use

   Tags

   8his tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO-3xFLAG-His6-LARP1

   Plasmid

   #233626

   Purpose

   Expression of Flag- and His-tagged LARP1

   Depositor

   Shintaro Iwasaki

   Article

   Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

   Insert

   LARP1 (LARP1 Human)
   

   Use

   Tags

   3FLAG-His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

   Plasmid

   #182651

   Purpose

   human CD81-TEV cleavage site-FKBP-SmBiT tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)
   

   Use

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TetO-U2B''-fSNAPc

   Plasmid

   #106988

   Purpose

   open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   UB2" (SNRPB2 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-MUC2 D1 with cleavable His tag

   Plasmid

   #181813

   Purpose

   Expresses human MUC2 D1 with cleavable His tag, residues 21-389

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc2 D1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

   Plasmid

   #141184

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Has Service

   Cloning Grade DNA

   Insert

   S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
   

   Use

   Tags

   Signal peptide from influenza HA and Superfolder …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FLAG-CRBN-pGK-HYG

    Plasmid

    #107380

    Purpose

    Mammalian expression of FLAG-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225245

    Purpose

    Mammalian expression of iPASnFR-H in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

    Plasmid

    #225462

    Purpose

    Express mEGFP-fusion protein of FMR1 isoform 1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 isoform 1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 (+) -myc-OGA(GS-544)C181

    Plasmid

    #193996

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid

    #162280

    Purpose

    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN8A Variant 1, stabilized (SCN8A Human)
    

    Use

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    Modified human HSPA5 intron 4 inserted after bp23…

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-LoxP-mKate-LoxP-EGFP

    Plasmid

    #167940

    Purpose

    CMV promoter-LoxP-mKate-LoxP-EGFP

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    LoxP-mKate-LoxP-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO_Vim-SNAP-tag2

    Plasmid

    #226518

    Purpose

    Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    Vimentin-SNAP-tag2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.2/V5-DEST CDC42EP1 S192A

    Plasmid

    #69820

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S192A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge