We narrowed to 41,926 results for: tro
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Plasmid#73982PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.DepositorInsertEGFP-Otx2-shRNA2
UseRNAiExpressionMammalianPromoterCAGAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
ExpressionYeastMutationValine 163 changed to alanineAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn1b
Plasmid#61874Purposebacterial expression of a GST-Sesn1b (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRExpressionWormAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TC393
Plasmid#122625PurposePser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
ExpressionWormAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-CrxshRNA
Plasmid#73987PurposeCrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Crx gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKC123
Plasmid#134240Purposeexpresses gRNA targeting CENP A 3'DepositorInsertCENP A 3' CRISPR construct
UseCRISPRExpressionMammalianAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
ExpressionMammalianPromoterCAGAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPRmTmG2
Plasmid#69992PurposeThe CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.DepositorInsertgRNA that targets near LoxP sites
Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5
Plasmid#104844Purposeb2AR-SPARK TF componentDepositorInsertAVPR2-eLOV-TEVcs-FLAG-GAL4-V5
UseAAVExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTL472
Plasmid#69882PurposedCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassetteDepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag-Sesn2
Plasmid#61871PurposeLentivirial expression of Flag-Sesn2 (mouse)DepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR-puro-Sec5
Plasmid#31117DepositorAvailable SinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM149
Plasmid#134178PurposeBacterial expression of his-CENP-C 510-endDepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJP102
Plasmid#176480PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoterDepositorInsertsactin > NatR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP103
Plasmid#176481PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertsactin > HygR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-microRNA 722-Dendra2
Plasmid#97130Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 722 Dendra2 (mir722 Zebrafish)
UseZebrafishAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI. PGK-puro
Plasmid#136266PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletIExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pES002 (pTet-qacR wt)
Plasmid#69031Purposewild-type qacR downstream of a Tet promoterDepositorAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
721-2µ-staHIS3
Plasmid#40611DepositorAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK110
Plasmid#99535Purposecontains bgl operon sequence for E. coli in vitro transcription templateDepositorInsertE. coli bgl antisense region containing bglG
TagsNonePromoterlambda Pr promoter used for in vitro transcriptio…Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12CTermTAG
Plasmid#125267PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertCTermTAG
TagsHis-TagPromoterTrcAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
TagsHis-tagExpressionBacterialPromotertrc promoterAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-eGFP
Plasmid#136098PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_eGFP
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-mTurquoise2
Plasmid#136100PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_mTurquoise2
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS12_eGFP-linker
Plasmid#136086PurposeFP for N-term fusion with a CTAG without linkerDepositorInsertCDS12_eGFP(noStop)-SerGly10xAlaSer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mScarletI-linker
Plasmid#136088PurposeFP for N-term fusion with a CTAG without linkerDepositorInsertCDS12_mScarletI(noStop)-SerGly10xAlaSer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-FLAG/MKRN1(1-369)
Plasmid#78753PurposeTo overexpress MKRN1(1-369) in Mammalian CellsDepositorAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-PLAGL2
Plasmid#133369PurposePiggybac vector for constitutive expressionDepositorInsertPLAGL2 (PLAGL2 Human)
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-RFP-miR-223
Plasmid#97148PurposemiR-223 expressionDepositorInsertmiR-223
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM0
Plasmid#59125PurposeFAK fret control sensor without kinase domain with no linkerDepositorInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-PDZ
Plasmid#127478Purposecoding sequence for synthetic zinc finger ZF-BCB-PDZDepositorInsertZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
UseCell-free transcription–translationTagsalpha 1-syntrophin PDZ domainPromoterT7Available SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR35
Plasmid#69256Purposephsp16.41::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.41Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-sgMDH1
Plasmid#72875PurposeThe lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEBDepositorInsertsgMDH1
UseLentiviralAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
vector plix 4.03
Plasmid#121130Purposeinducible expression in cellsDepositorInsertempty cassete for contol of miR expression
ExpressionBacterialAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only