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161,570 results

Showing: 11481 - 11520 of 161570 results

  3. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Promoter
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  6. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    tight TRE promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127372-plasmid-map-sequence-id-247988

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  10. TFORF3056

    Plasmid
    #144532
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    THRB (THRB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144532-plasmid-map-sequence-id-404212

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  12. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid
    #218513
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of the Plac_mrfp cassette
    Promoter
    PgyrA_ermBL-ermB'
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218513-plasmid-map-sequence-id-444102

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  15. iC13 PB-TO-oNgn2-BFP-puro

    Plasmid
    #204733
    Purpose
    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Insert
    Optimized neurogenin-2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    204733-plasmid-map-sequence-id-403381

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  18. qTAG-AAVS1-PGK-Blast

    Plasmid
    #227270
    Purpose
    Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227270-plasmid-map-sequence-id-452024

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  23. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid
    #52093
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52093-plasmid-map-sequence-id-80507

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  25. TFORF0338

    Plasmid
    #144261
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MECOM (MECOM Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144261-plasmid-map-sequence-id-404152

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  27. pSBY3_ligD-HygR

    Plasmid
    #104625
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104625-plasmid-map-sequence-id-201573

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  29. pHIV-Luciferase

    Plasmid
    #21375
    Purpose
    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21375-plasmid-map-sequence-id-357945

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  32. pCW57-GFP-P2A-MCS (Neo)

    Plasmid
    #89181
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89181-plasmid-map-sequence-id-176294

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  38. pNR220

    Plasmid
    #79541
    Purpose
    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    A118
    Use
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79541-plasmid-map-sequence-id-148753

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  40. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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Showing: 11481 - 11520 of 161570 results