We narrowed to 22,836 results for: Sis

1. pRH3153

   Plasmid

   #210173

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-L102P-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::ade1-L102P-GFP::tADH1 (ADE2 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   L102P

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRH3155

   Plasmid

   #210175

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-L32R-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::ade1-L32R-GFP::tADH1 (ADE2 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   L32R

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRH3218

   Plasmid

   #210186

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151N-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::lys1-W151N-GFP::tADH1 (LYS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   W151N

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRH3152

   Plasmid

   #210172

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-G54E-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::ade1-G54E-GFP::tADH1 (ADE2 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   G54E

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRH3069

   Plasmid

   #210163

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1 (LYS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   W151V

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRH3047

   Plasmid

   #210157

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1 (LYS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   W151H

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRH3055

   Plasmid

   #210161

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151Q-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::lys1-W151Q-GFP::tADH1 (LYS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   W151Q

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRH3048

   Plasmid

   #210158

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151Y-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::lys1-W151Y-GFP::tADH1 (LYS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   W151Y

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRH3053

   Plasmid

   #210159

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1 (LYS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   W151A

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYea5.1_Notch1_sfGFP

    Plasmid

    #207801

    Purpose

    Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein
    

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Promoter

    TEF

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB(EXPR-PYL-DNMT1-NEO)

    Plasmid

    #114396

    Purpose

    For PYL-DNMT1 expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-DNMT1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 NKX6-2-Venus R200W

    Plasmid

    #209179

    Purpose

    Mammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent protein

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (R200W) fused with Venus (NKX6-2 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (R200W)

    Promoter

    CMV

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 NKX6-2-Venus L163V

    Plasmid

    #209178

    Purpose

    Mammalian expression of SPAX8-related missense mutation (L163V) in human NKX6-2 fused with the Venus fluorescent protein

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (L163V) fused with Venus (NKX6-2 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (L163V)

    Promoter

    CMV

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmcb306-GFP-Rab12-mito

    Plasmid

    #207349

    Purpose

    Mammalian expression of Rab12-mito

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    Rab12-mito (RAB12 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-HisD-BR1

    Plasmid

    #192816

    Purpose

    Expresses BR1 in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    BR1 (BRI1 Mustard Weed)
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS506

    Plasmid

    #200712

    Purpose

    Promoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeast

    Depositor

    Jens Boch

    Article

    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

    Insert

    PMA1 promoter (PMA1 Budding Yeast)
    

    Use

    Synthetic Biology; Modular cloning

    Mutation

    domesticated for MoClo cloning (removal of intern…

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCitro-15a

    Plasmid

    #202033

    Purpose

    p15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter

    Depositor

    David Edgell

    Article

    Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

    Insert

    conjugative system from a Citrobacter spp.
    

    Use

    Synthetic Biology

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eSrtA-MT4

    Plasmid

    #200306

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT4 (MT4 Human)
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-THTAP-SPARTIN, residues 1-101

    Plasmid

    #180626

    Purpose

    Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPARTIN
    

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-101

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-THTAP-CALPAIN-7 1-165

    Plasmid

    #180610

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-65

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCA528 CALPAIN-7 residues 1-165

    Plasmid

    #180611

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUBQ10:LYK5_TM-3xHA

    Plasmid

    #202191

    Purpose

    Express citrus 'Tango' mandarin LYK5 gene under the UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    LYSM-CONTAINING RECEPTOR-LIKE KINASE 5
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUBQ10:WNFLS2-2

    Plasmid

    #202190

    Purpose

    Express 'Washington navel' Orange FLS2-2 gene under the UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUBQ10:WNFLS2-1

    Plasmid

    #202189

    Purpose

    Express 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-TC9

    Plasmid

    #202088

    Purpose

    entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

    Depositor

    David Edgell

    Article

    Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

    Insert

    TevCas9 + sgRNA cassette
    

    Use

    CRISPR

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-RIPK3[K5A]-FLAG

    Plasmid

    #199326

    Purpose

    mutated ubiquitination site (ubiquitination inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 5 to Alanine

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-RIPK3[K5R]-FLAG

    Plasmid

    #199325

    Purpose

    mutated ubiquitination site (ubiquitination active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 5 to Arginine

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SUMO_LPETG_MT3

    Plasmid

    #200304

    Purpose

    Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Tags

    Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJL1-MBP-DQNAT

    Plasmid

    #198215

    Purpose

    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    MBP_DQNAT
    

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJL1-PD-DQNAT

    Plasmid

    #198216

    Purpose

    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    PD-DQNAT
    

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYUBcLIC-GFP

    Plasmid

    #183509

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Promoter

    T7 lac

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

    Plasmid

    #200124

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

    Plasmid

    #200125

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNL2114 pARF19 GG0-GG1

    Plasmid

    #195909

    Purpose

    Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pARF19 (ARF19 Mustard Weed)
    

    Use

    Synthetic Biology

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pWzl_CRKL-W160L+W275L

    Plasmid

    #196021

    Purpose

    Expresses CRKL W160L + W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-EGFP-DmMe31B-E208Q_E

    Plasmid

    #146196

    Purpose

    Insect Expression of DmMe31B-E208Q

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-E208Q (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAc5.1B-EGFP-DmMe31B-K108A_E

    Plasmid

    #146197

    Purpose

    Insect Expression of DmMe31B-K108A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-K108A (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1B-EGFP-DmMe31B-mut1_E

    Plasmid

    #146198

    Purpose

    Insect Expression of DmMe31B-mut1

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-mut1 (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmMe31B-E208Q_E

    Plasmid

    #146201

    Purpose

    Insect Expression of DmMe31B-E208Q

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-E208Q (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-DmMe31B-K108A_E

    Plasmid

    #146202

    Purpose

    Insect Expression of DmMe31B-K108A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-K108A (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge