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2,366 results

Showing: 561 - 580 of 2366 results

  4. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

    Plasmid
    #154781
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    CRFR1 (Crhr1 Rat)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …
    Promoter
    EF1
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154781-plasmid-map-sequence-id-310400

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  5. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid
    #196223
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196223-plasmid-map-sequence-id-405486

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  7. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104492
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104492-plasmid-map-sequence-id-381181

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  10. pGFP Cas

    Plasmid
    #50729
    Purpose
    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Cas (Bcar1 Rat)
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50729-plasmid-map-sequence-id-340937

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  11. pDS170-enNTS1

    Plasmid
    #199152
    Purpose
    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)
    Depositor
    Daniel Scott
    Article
    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:
    Insert
    enNTS1 (Ntsr1 Rat)
    Tags
    10xHis tag, Avi tag, MBP, and muGFP
    Expression
    Bacterial
    Mutation
    V208M, C273S, C391S and C393S (over NTS1-B5)
    Promoter
    Lac/T5
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199152-plasmid-map-sequence-id-393468

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  12. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid
    #102996
    Purpose
    Expresses synaptophysin fused GCaMP6f under the synapsin promoter
    Depositor
    David Cox
    Insert
    Synaptophysin-GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    102996-plasmid-map-sequence-id-218069

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  13. pGL3-alphaMHC 3300bp

    Plasmid
    #51934
    Purpose
    luciferase reporter for rat alpha-MHC promoter
    Depositor
    Jeffery Molkentin
    Insert
    alpha MHC (Myh6 Rat)
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    -2930 to +410 promoter region
    Promoter
    alpha MHC
    Available Since
    May 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104496
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    104496-plasmid-map-sequence-id-474734

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  16. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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Showing: 561 - 580 of 2366 results