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2,384 results

Showing: 561 - 580 of 2384 results

  1. pCMV-dCpf1-eBE-YE

    Plasmid
    #107689
    Purpose
    Expresses dLbCpf1-eBE-YE in mammalian cells
    Depositor
    Jia Chen
    Publication
    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-eBE-YE
    Expression
    Mammalian
    Mutation
    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)
    Promoter
    CMV
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    107689-plasmid-map-sequence-id-212121

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  2. pCMV-dCpf1-BE-YE

    Plasmid
    #107686
    Purpose
    Expresses dLbCpf1-BE-YE in mammalian cells
    Depositor
    Jia Chen
    Publication
    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-BE-YE
    Expression
    Mammalian
    Mutation
    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)
    Promoter
    CMV
    Available since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    107686-plasmid-map-sequence-id-207597

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  3. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107737
    Purpose
    In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107737-plasmid-map-sequence-id-207281

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  6. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55776-plasmid-map-sequence-id-112541

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  7. pBa-FRB-3myc-KIF1B beta tail 387-1770

    Plasmid
    #64287
    Purpose
    KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags
    Depositor
    Gary Banker, Marvin Bentley
    Publication
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1B beta (Kif1b Mouse, Rat)
    Tags
    3myc and FRB
    Expression
    Mammalian
    Mutation
    aa 387-1770, small portion of 5'end matches …
    Promoter
    chicken Beta Actin
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    64287-plasmid-map-sequence-id-328082

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  9. mPlum-Lysosomes-20

    Plasmid
    #55982
    Purpose
    Localization: Lysosome Membrane, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1 (Lamp1 Rat)
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    D50E
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55982-plasmid-map-sequence-id-147991

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  10. VAMP2-12aa-mCit

    Plasmid
    #53237
    Purpose
    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    12aa-linker and mCitrine
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Publication
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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Showing: 561 - 580 of 2384 results