We narrowed to 4,893 results for: PIR;

1. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221076

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221077

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221079

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK-caCas9-NatMX-Neut5L-Leu2 drive

   Plasmid

   #89578

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Leu2 gene drive
   

   Expression

   Yeast

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT C-His Ana GvpC in pET28a

   Plasmid

   #85732

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (his-tagged)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

   Plasmid

   #221080

   Purpose

   SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

   Plasmid

   #221082

   Purpose

   Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfGFP[r5M]_ACC-mEBFP2
   

   Use

   Synthetic Biology

   Promoter

   CMV, T7

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. N370S GBA pcDNA3.1

   Plasmid

   #188583

   Purpose

   Ectopic expression N370S GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Mutation

   N370S

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. L444P GBA pcDNA3.1

   Plasmid

   #188582

   Purpose

   Ectopic expression L444P GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Mutation

   L444P

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. E326K GBA pcDNA3.1

    Plasmid

    #188581

    Purpose

    Ectopic expression E326K GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Expression

    Mammalian

    Mutation

    E326K

    Promoter

    CMV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK-caCas9-NatMX-Neut5L-Ade2 drive

    Plasmid

    #89577

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Ade2 gene drive
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAP05

    Plasmid

    #186261

    Purpose

    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Expression

    Bacterial

    Promoter

    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-MEK1

    Plasmid

    #68300

    Purpose

    N-terminal MBP tagged human MEK1 gene for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 (MAP2K1 Human, Synthetic)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. C-His-GvpC-TEV

    Plasmid

    #153296

    Purpose

    Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    TEV-sensitive Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) 21 bp TEV recognition and cleavage site flanke…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. N-His-GvpC-ssrA

    Plasmid

    #153297

    Purpose

    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    

    Tags

    His-tag and ssrA tag

    Expression

    Bacterial

    Mutation

    1) N-terminal 6xHis tag flanked by 1x glycine res…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5.1B-EGFP-DmCYFIP1_T

    Plasmid

    #147578

    Purpose

    Insect Expression of DmCYFIP1

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmCYFIP1 (Sra-1 Fly)
    

    Expression

    Insect

    Mutation

    5 silent and one non silent A146V mutations compa…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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