Addgene

We narrowed to 1,006 results for: hug;

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pcDNA-K-GECO1

Plasmid

#105865

Purpose

Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Use

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Gateway entry clone

Tags

KDEL retention signal and signal peptide

Expression

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for caspase-1

Plasmid

#60885

Purpose

FPX biosensor for caspase-1

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-1

Use

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-BaeI-Pgk-Cre

Plasmid

#173663

Purpose

Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

BaeI site

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.3#3/Cre

Plasmid

#173662

Purpose

Expresses a NT.3-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171615

Purpose

Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for caspase-8

Plasmid

#60884

Purpose

FPX biosensor for caspase-8

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-8

Use

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTR AA Clover

Plasmid

#90238

Purpose

Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AA mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC6-METAP1-3xFLAG

Plasmid

#163453

Purpose

lentiviral overexpression vector for human METAP1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

METAP1 (METAP1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGLS_2

Plasmid

#163459

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting GLS (GLS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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J-sRNA-331Bb

Plasmid

#78285

Purpose

An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

j-sRNA-331

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-K-GECO1

Plasmid

#105864

Purpose

Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Use

Tags

6-His Tag

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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