We narrowed to 18,122 results for: URE

Showing: 8671 - 8700 of 18122 results

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mCherry-Clathrin LC-15

Plasmid

#55019

Purpose

Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin LC (CLTB Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Article

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-MFSD12-V5

Plasmid

#149508

Purpose

Stable expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Lentiviral

Tags

V5

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKRaION1-N1

Plasmid

#177801

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION1

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

Plasmid

#46670

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-let-7a-1 (MIRLET7A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-flag-Lag16-iCAM-1

Plasmid

#205195

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28sumo-hRig-I-Full-length

Plasmid

#210827

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

Insert

hRIG-I (DDX58) Full length protein (RIGI Human)

Expression

Bacterial

Mutation

NA

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)

Plasmid

#166835

Purpose

Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Type

Empty backbone

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-fDIO-mNeonGreen

Plasmid

#241240

Purpose

FRT-dependent expression of fluorescent protein, mNeonGreen, in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

fDIO-mNeonGreen

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Article

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Expression

Bacterial

Promoter

glnS' and lambda(ts) and proK

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-N

Plasmid

#210270

Purpose

N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Claspin-Flag

Plasmid

#136246

Purpose

Protein expression of Claspin with a C terminal FLAG tag

Depositor

Aziz Sancar

Article

Sar et al J Biol Chem. 2004 Sep 17;279(38):39289-9

Insert

Claspin

Tags

Flag

Expression

Mammalian

Mutation

E263G, Q499R, I783T, D932G and S1062G- Please see…

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_N_Strep_BIRC6

Plasmid

#197972

Purpose

Mammalian cell expression of wt Strep-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

Strep

Expression

Mammalian

Mutation

L1332V

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPD0011_Luc-P2A-Zeo

Plasmid

#242534

Purpose

Cell line generation

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

Firefly lucifrease

Use

Lentiviral

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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NP-11-64-1

Plasmid

#234359

Purpose

Expressing rubisco and prk in Δrpi

Depositor

David Savage

Article

Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

R.rubrum rubisco

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pECIA2

Plasmid

#47032

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Fc(human IgG1), HRV 3C Protease Cleavage site, He…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc

Plasmid

#176049

Purpose

Expresses Myc tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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hSirt3-RFP

Plasmid

#216806

Purpose

Human Sirt3-RFP driven by the CaMKII promoter

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (SIRT3 Human)

Use

Lentiviral

Tags

mRFP1

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRFP-NLS

Plasmid

#105296

Purpose

To visualize nuclear dynamics in living cells

Depositor

Kenneth Yamada

Article

Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

Insert

Rac1 (RAC1 Human)

Tags

RFP

Expression

Mammalian

Mutation

aa encoding 172-192

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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