We narrowed to 164,546 results for: HTT

1. LEMD3_BirA_Nterm

   Plasmid

   #221518

   Purpose

   Flag-tagged biotinylation enzyme for co-expression of Lemd3

   Depositor

   Alessandro Ori

   Insert

   LEMD3 (LEMD3 Human)
   

   Use

   Tags

   BirA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mPlum-MyosinIIA-C-18

   Plasmid

   #55989

   Purpose

   Localization: Cytoskeleton, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   MyosinIIA (Myh9 Mouse)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-myc-Cdc42-wt

   Plasmid

   #12972

   Depositor

   Gary Bokoch

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-5'TwStrep

   Plasmid

   #208620

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NEDD4

   Plasmid

   #25141

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   nedd4.0001.0152 (NEDD4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. R777-E143 Hs.NFKB1

   Plasmid

   #70427

   Purpose

   Gateway ORF clone of human NFKB1 [NM_003998.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

   Plasmid

   #160629

   Purpose

   TU for the expression of LbCas12a

   Depositor

   Diego Orzaez

   Insert

   AtUBQ10-:LbCpf1(Cas12a):Tnos
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   UBQ10

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA

   Plasmid

   #20296

   Purpose

   Cre-activated AAV expression of EYFP

   Depositor

   Karl Deisseroth

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SEC14L3

   Plasmid

   #73240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SEC14L3 (SEC14L3 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

10. SEC14L4

    Plasmid

    #73233

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SEC14L4 (SEC14L4 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEGFP-TPX2-N-10

    Plasmid

    #56289

    Purpose

    Localization: Centrosomes, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    TPX2 (TPX2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pGEX-2T-RhoA-T19N

    Plasmid

    #12960

    Depositor

    Gary Bokoch

    Insert

    RhoA DN (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T19N Dominant negative

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid

    #186046

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGRB2.3

    Plasmid

    #45343

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Tags

    Expression

    Mutation

    Promoter

    PGK1

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS L30 mRuby3-Rab10

    Plasmid

    #166429

    Purpose

    Rab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab10
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid

    #183533

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-dSa-VPR

    Plasmid

    #99651

    Purpose

    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

    Depositor

    George Church

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65-RTA activator

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-tMCK-mem-TurboID

    Plasmid

    #149410

    Purpose

    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Insert

    mem-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-hUCP4-NE

    Plasmid

    #102362

    Purpose

    Overexpression of solute carrier family 25 member 27 in mammalian cells

    Depositor

    Shu Leong Ho

    Insert

    solute carrier family 25 member 27 (SLC25A27 Human)
    

    Use

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAIP4_4WDC

    Plasmid

    #234153

    Purpose

    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4WDC
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pRSII423

    Plasmid

    #35464

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast 2 micron vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEmerald-Coronin1B-C-10

    Plasmid

    #54049

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Coronin1B (CORO1B Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pF

    Plasmid

    #42519

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Type

    Empty backbone

    Use

    Unspecified; Cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, T3

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-MS2-HA-Hs4E-T_AA

    Plasmid

    #148233

    Purpose

    Mammalian Expression of Hs4E-T

    Depositor

    Elisa Izaurralde

    Insert

    Hs4E-T (EIF4ENIF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p405TEF1

    Plasmid

    #15968

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    TEF1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET3a-Yuh1

    Plasmid

    #18895

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

28. R777-E035 Hs.DUSP2

    Plasmid

    #70319

    Purpose

    Gateway ORF clone of human DUSP2 [NM_004418.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    DUSP2 (DUSP2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAS9i_TRP

    Plasmid

    #141253

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-H2B-6

    Plasmid

    #56436

    Purpose

    Localization: Nucleus/Histones, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. PRS_8-jGCaMP7b

    Plasmid

    #192594

    Purpose

    AAV-vector for fluorescent calcium measurements from noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Maheu et al bioRxiv 2025.03.26.644382

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid

    #51656

    Purpose

    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

    Depositor

    Adam Cohen

    Insert

    Quasar2-mOrange-Chr64-GFP
    

    Use

    Lentiviral

    Tags

    GFP and mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSII415

    Plasmid

    #35454

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast centromere vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MDC1 in pENTR4 (330-5)

    Plasmid

    #26427

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RAB1A

    Plasmid

    #25521

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

36. pN1-pHlu

    Plasmid

    #129315

    Purpose

    pH measurement

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    pHluorin (from H. Imamura)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Bsrs078-TraR(W)

    Plasmid

    #85151

    Purpose

    Constitutively expresses TraR(W)

    Depositor

    Jeff Hasty

    Article

    Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

    Insert

    TraR(W)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS-L1PA1CH_blast rescue

    Plasmid

    #69611

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Use

    Tags

    blast rescue cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 362 pCS Cherry DEST

    Plasmid

    #13075

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    Cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DFNB31-TwinStrepII

    Plasmid

    #83196

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    DFNB31 (Whrn Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge