We narrowed to 10,745 results for: AGA

1. pSLQ1373_mU6_sgRNA targeting e3

   Plasmid

   #190685

   Purpose

   sgRNA targeting enhancer 3 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 3 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGE_En03-sgRNA_Target1 (Mpphot)

   Plasmid

   #186726

   Purpose

   Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

   Depositor

   Yutaka Kodama

   Article

   Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

   Insert

   sgRNA_Mphot
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLiP-G2

   Plasmid

   #188964

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBO.ACT004

   Plasmid

   #182714

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB9342 (pgRNA_XIII-1_NatMX)

   Plasmid

   #161594

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB9344 (pgRNA_XVI-1_NatMX)

   Plasmid

   #161596

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cherry-Plekhh1 5 silent mutations

   Plasmid

   #187373

   Purpose

   Expresses human Plekhh1 with 5 silent mutations labelled with Cherry

   Depositor

   Evelyne Coudrier

   Article

   Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

   Insert

   Plekhh1 with 5 silent mutations
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Five silent mutations at the Plekhh1 siRNA site (…

   Promoter

   CMV

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1056J

   Plasmid

   #183136

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1056K

    Plasmid

    #183137

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

    Plasmid

    #179006

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

    Plasmid

    #178999

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPPC030.306

    Plasmid

    #171151

    Purpose

    Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-mvaES
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-GFP-mirNega

    Plasmid

    #163705

    Purpose

    pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

    Depositor

    Michisuke Yuzaki

    Article

    Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

    Insert

    mirNega and EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-mSNX27-H112A RNAi-resistant

    Plasmid

    #163621

    Purpose

    mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H112A and RNAi resistance

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKO.1-shSpc25

    Plasmid

    #160960

    Purpose

    Spc25 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Spc25 shRNA (Spc25 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-U6a-U6c-mfn2 guide#2

    Plasmid

    #121994

    Purpose

    An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mfn2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p3E-U6a-U6c-opa1 guide

    Plasmid

    #121995

    Purpose

    An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    opa1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BII-gR-PnGW

    Plasmid

    #133355

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

    Plasmid

    #128758

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #3 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

    Plasmid

    #128756

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. gh264

    Plasmid

    #106863

    Purpose

    expression of gRNA targeting ALG10B

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ALG10B (ALG10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gh181

    Plasmid

    #106842

    Purpose

    expression of gRNA targeting GYG2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GYG2 (GYG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. gamma2 CR3m shRNA

    Plasmid

    #120795

    Purpose

    Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gamma2 UTR3m shRNA

    Plasmid

    #120797

    Purpose

    Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAPM-D4 miR30-SUV39h2 ts1

    Plasmid

    #115883

    Purpose

    SUV39h2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h2 shRNA (SUV39H2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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