164,223 results

1. HA Depdc5 pRK5

   Plasmid

   #46327

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Differs from Depdc5 isoform 1 by 10 amino acids …

   Promoter

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-CMV-Puro-PNPT1

   Plasmid

   #223315

   Purpose

   Lentiviral vector expressing human PNPT1

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CT752

   Plasmid

   #233658

   Purpose

   Flp GFP Reporter; FRT-STOP-FRT-GFP

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   FRT-STOP-FRT-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CT769

   Plasmid

   #233661

   Purpose

   Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   PhyA-Flp-397-Flp-396-FHY1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_WT RAD23A

   Plasmid

   #201438

   Purpose

   Expresses human RAD23A with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL4723-P1

   Plasmid

   #86173

   Purpose

   LevelP cloning Vector

   Depositor

   Mark Youles

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. SGEP

   Plasmid

   #111170

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-ER GFP

    Plasmid

    #80069

    Purpose

    To express and target GFP into the endoplasmic reticulum using lentivirus

    Depositor

    Pantelis Tsoulfas

    Insert

    ER GFP (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    GFP derived from Aequorea coerulescens on the NH2…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-GLUT4-7myc-GFP

    Plasmid

    #52872

    Purpose

    detection and measurement of GLUT4 translocation

    Depositor

    Jonathan Bogan

    Article

    Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

    Insert

    GLUT4 (Slc2a4 Rat, with human c-terminus)
    

    Use

    Retroviral

    Tags

    7x-myc (internal) and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1413I

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF(UG) hSyn LAMP1-msGFP (JV012)

    Plasmid

    #179728

    Purpose

    Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    LAMP1 (Lamp1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHiUGE GS-gRNA (hSyn-Cre) mTubb3

    Plasmid

    #200369

    Purpose

    Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Mouse Tubb3 gRNA and Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-TRE-U2AF2-TurboID-HA-rtTA

    Plasmid

    #226997

    Purpose

    Integrative plasmid to express U2AF2-TurboID in mammalian cells

    Depositor

    Bruno Dallagiovanna

    Article

    Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

    Insert

    U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
    

    Use

    Tags

    TurboID-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Blast-dTAG-LMNB1

    Plasmid

    #207776

    Purpose

    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid

    #172507

    Purpose

    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pCMV - huDisCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-WASH1-C-18

    Plasmid

    #55162

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    WASH1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

20. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Expression

    Mutation

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. 401 pSG5L

    Plasmid

    #10737

    Depositor

    William Sellers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ePB-PURO-TT-PA-CRE

    Plasmid

    #179584

    Purpose

    ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    PA-MAG-CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGS-6011nes

    Plasmid

    #108652

    Purpose

    An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-ERK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. CL20_mEGFP_FUS_FLI1

    Plasmid

    #205822

    Purpose

    Express mEGFP-tagged fusion protein, FUS_FLI1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    FUS_FLI1 (FUS, FLI1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

    Plasmid

    #134662

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 5 (VAMP5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHM6-alphasynuclein-WT

    Plasmid

    #40824

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. huCaV2.2 (pSAD442-1)

    Plasmid

    #62574

    Purpose

    Human CaV2.2 expression in mammalian cells

    Depositor

    Diane Lipscombe

    Article

    Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

    Insert

    CACNA1B (CACNA1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLIK-PUM1-rABE-Flag

    Plasmid

    #191385

    Purpose

    Doxycycline inducible expression of PUM1-rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    PUM1-rABE (PUM1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    tet-inducible promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge