We narrowed to 9,894 results for: UTY

1. pAAV-CAG-FLEx-iSeroSnFR

   Plasmid

   #128486

   Purpose

   Fluorescent reporter for serotonin (conditional + membrane localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Has Service

   AAV9

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Ig-kappa leader, Myc, PDGFR, and cpsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSAD-5PSD95-EGFP-SynPhRFP

   Plasmid

   #217979

   Purpose

   G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

   Depositor

   Edward Callaway

   Article

   Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

   Inserts

   5PSD95-EGFP (Dlg4 Rat)
   SynPhRFP (Syp Mouse)
   

   Use

   G-deleted rabies

   Tags

   EGFP and RFP

   Expression

   Mutation

   Promoter

   None

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRFP-Vimentin-17-C

   Plasmid

   #108414

   Purpose

   Vimentin fused to monomeric near-infrared fluorescent protein miRFP

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   miRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85806

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   CMV

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

   Plasmid

   #185706

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   Cas9
   

   Use

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINO_H3

   Plasmid

   #171927

   Purpose

   Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

   Insert

   VHH_H3
   

   Use

   Tags

   His6

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLenti GW V5 Eco/Dam hum LaminA

   Plasmid

   #182673

   Purpose

   Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

   Depositor

   Bas van Steensel

   Article

   Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   E. Coli Dam and V5

   Expression

   Mammalian

   Mutation

   Promoter

   HSP

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

   Plasmid

   #156402

   Purpose

   Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4B-WT (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKII-Archon1-KGC-EGFP-ER2

   Plasmid

   #108419

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223167

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112214

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mutation

    D10A,H840A,N863A

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. px335 Mettl14 sgRNA #2

    Plasmid

    #61514

    Purpose

    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    gccgctcccggatctcctgc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pOPINO_C5

    Plasmid

    #171925

    Purpose

    Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_C5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112210

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNA methyltransferase 3 alpha (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A,H840A,N863A

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112209

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A,H840A,N863A,C710S

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINO_C1

    Plasmid

    #171924

    Purpose

    Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_C1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

    Plasmid

    #156411

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET.SUMO eIF4G1 1-200

    Plasmid

    #158791

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (codon optimized)
    

    Use

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-Archon2-KGC-EGFP-ER2

    Plasmid

    #108421

    Purpose

    AAV production plasmid encoding for Archon2 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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