178,218 results
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Plasmid#121944PurposeCRISPR-Sirius plasmidDepositorInserthU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6 promotersAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSI-Target-AID-NG
Plasmid#119861PurposeExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
ExpressionMammalianAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCWX-UBI-hTert-PGK-BSD
Plasmid#114316PurposeConstitutive expression of the hTert proteinDepositorInserthTERT (TERT Human)
UseLentiviralAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_RTX_(exo-)
Plasmid#102786PurposeExpresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)DepositorInsertreverse transcription xenopolymerase with 3'-5' deficient exonuclease
ExpressionBacterialAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP
Plasmid#111581PurposeAn AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
ExpressionBacterialAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 2 kb prom. CD274
Plasmid#107003PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-3xFLAG-TEV-UBE2O
Plasmid#105718PurposeExpresses WT full length human N- terminally tagged UBE2ODepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-5.5kb-UCP1-GFP
Plasmid#104585PurposeReporter assay to monitor the transcription activity of the UCP1 promoter by GFP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUCP1 promoter (Ucp1 Mouse)
TagsTurboGFPExpressionMammalianPromoterUCP1Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJC5-LAMP1-RFP-2xFlag
Plasmid#102931PurposeLentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)DepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneTagsSNAP-tag (SNAP26b)ExpressionBacterialAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
B52 (empty plasmid backbone to express 2 sgRNAs)
Plasmid#100708PurposeEmpty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRN11
Plasmid#84455Purposeshuttle plasmid for sarA P1-mCherry expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
UseShuttle vector e.coli-s.aureus, expression of sgf…Available sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MICU-APEX2
Plasmid#79057PurposeA C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1DepositorInsertC-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
UseLentiviralTagsFLAGExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH-sgRNA1-2XPP7
Plasmid#75390PurposesgRNA1-2XPP7DepositorInsertsgRNA1-2XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferasePromoterVEGF -1000 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposeExpression of membrane-tethered EGFP and Synaptophysin-mRuby from Flp-expressing neuronsDepositorInsertsEGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by