We narrowed to 22,677 results for: Sis;

1. Halo-WIPI2 HRD

   Plasmid

   #207544

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human WIPI2 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVP2A-PIF6

   Plasmid

   #73369

   Purpose

   Expresses Adeno-associated virus VP2 with PIF6 insert.

   Depositor

   Junghae Suh

   Article

   Gomez et al ACS Nano. 2015 Nov 30.

   Insert

   PIF6 (PIL2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P33

   Plasmid

   #120018

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SHC003BSD-DelGFP

   Plasmid

   #133301

   Purpose

   EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. hOC-GFPtpz

   Plasmid

   #110207

   Purpose

   Expresses GFP in mature bone cells.

   Depositor

   David Rowe

   Article

   Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

   Insert

   eGFPtopaz
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Human osteocalcin (BGLAP)

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23a-HsRPS27a

   Plasmid

   #69561

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDEST_6xUAS:miRFP670-cetn4

   Plasmid

   #192357

   Purpose

   zfCETN4 tagged with miRFP670, expressed under the UAS promoter

   Depositor

   Darren Gilmour

   Article

   Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

   Insert

   CETN4 (cetn4 Zebrafish)
   

   Use

   Tags

   miRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLAS3W-S100P

   Plasmid

   #125009

   Purpose

   express in mammalian cell

   Depositor

   Ruey-Hwa Chen

   Article

   Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

   Insert

   S100P (S100P Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p415TEF cyto roGFP2-Grx1(yeast)

   Plasmid

   #65004

   Purpose

   Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

   Insert

   Glutaredoxin-1
   

   Use

   Tags

   roGFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26_MraYaa

    Plasmid

    #100166

    Purpose

    recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Chung et al Science. 2013 Aug 30;341(6149):1012-1016

    Insert

    mraY (phospho-MurNAc-pentapeptide translocase)
    

    Use

    Tags

    MBP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT44c

    Plasmid

    #138511

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9-UGI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Col1a-3'probe

    Plasmid

    #20731

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1a-3'probe (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMADM-beta

    Plasmid

    #36891

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    Beta-Geo
    thymidine kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-myc-hIFITM3

    Plasmid

    #58461

    Purpose

    Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pUPD2_miniDFR (GB2566)

    Plasmid

    #160595

    Purpose

    Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

    Depositor

    Diego Orzaez

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    miniDFR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMaCTag-P27

    Plasmid

    #120038

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU53

    Plasmid

    #167679

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMaCTag-Z22

    Plasmid

    #120065

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD_C'5802

    Plasmid

    #176879

    Purpose

    High constitutive expression of Firefly Luciferase in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    AtUbi10:Luc+:RbcsE9
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18-H1 RNAi

    Plasmid

    #87355

    Purpose

    RNAi expression vector based on H1 promoter and pUC18 vector backbone

    Depositor

    Saroj Mathupala

    Article

    Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

    Insert

    H1 RNA Promoter from U87MG glioma cells
    

    Use

    RNAi; Shrna expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 RNA

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-DUET-1-mRhubarb720-HO

    Plasmid

    #141201

    Purpose

    Expresses mRhubarb720 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb720
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, L196Q, T202D, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSG5-FER

    Plasmid

    #30191

    Depositor

    Nora Heisterkamp

    Article

    Hao et al Mol Cell Biol. 1989 Apr . 9(4):1587-93.

    Insert

    Fer tyrosine kinase (FER Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pCDFDuet-trAtCCD7-OsD27(pYL726 )

    Plasmid

    #178285

    Purpose

    Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    OsD27
    trAtCCD7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pR-mClover3

    Plasmid

    #228605

    Purpose

    Mammalian expression of mClover3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EFF-1::V5

    Plasmid

    #132961

    Purpose

    Expresses EFF-1 A full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    EFF-1::V5
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL3-VEGFR2-225

    Plasmid

    #21306

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pET-E(4130)A2

    Plasmid

    #168021

    Purpose

    Expresses E(4130)A2-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    E(4130)A2-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mEos4a-C1

    Plasmid

    #54810

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4a

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pMaCTag-Z34

    Plasmid

    #120062

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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