We narrowed to 24,925 results for: crispr
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Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B9
Plasmid#172850PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pARNTL.1.0-gDNA
Plasmid#112480PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTLDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertARNTL (BMAL1 Human)
UseCRISPRExpressionMammalianAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUNB.1.0-gDNA
Plasmid#112409PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJUNB (JUNB Human)
UseCRISPRExpressionMammalianAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro
Plasmid#227266PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1
Plasmid#194279PurposeExpresses gRNA and VP64-dSaCas9-VP64 from lentiviral vectorDepositorInsertshumanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhU6 and hUbCAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1
Plasmid#194278PurposeExpresses gRNA and dSaCas9-KRAB from lentiviral vectorDepositorInsertshumanized dSaCas9 KRAB T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhU6 and hUbCAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-CMV-NLS-dCas9-NLS
Plasmid#99908PurposeFor the expression of NLS-dCas9-NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-dCas9-NLS
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
ACE2 g3
Plasmid#153013PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherryDepositorAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_EF1a_CasRx_Hygro
Plasmid#134841PurposeLentiviral all in one expression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPR and LentiviralTagsT2A HygromycinExpressionMammalianPromoterEF1aAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Homology Arms-BRD4-HaloTag
Plasmid#228575PurposeCRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertionDepositorAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.tRFP
Plasmid#169941PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKDsgRNA-pgaC
Plasmid#154919PurposeaTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pgaC
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpTetAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMLS257
Plasmid#73716PurposeSapTrap destination vector for building repair template only vectorsDepositorInsertSapI repair template insertion site
UseCRISPRAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRosa26-EF1a-hCas9IRESneo
Plasmid#67987PurposeMouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassetteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEF1a-hCas9IRESneopA
ExpressionMammalianAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W
Plasmid#67983PurposeCas9 activity reporter (control) with GFP and BFPDepositorInsertU6gRNA cassette, PGKGFP2ABFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRH623
Plasmid#171633Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-non-targeting
mNeon
UseCRISPR and LentiviralExpressionMammalianPromoterEF1-a and U6Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only