We narrowed to 10,337 results for: plasmids 101

1. pIC292

   Plasmid

   #51405

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska2 IMAGE 4685972 (SKA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM160

   Plasmid

   #134169

   Purpose

   Bacterial expression of RB3 stathmin-like domain (SLD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   RB3-SLD (STMN4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues 49-189

   Promoter

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. DNA-CARzeta-IRESpuro

   Plasmid

   #89345

   Purpose

   2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   DNA-CARzeta
   

   Use

   Lentiviral

   Tags

   SNAPf tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. POLArIS_NbVim-cp10GFP

   Plasmid

   #171020

   Purpose

   Expresses NbVim-cp10GFP#2 in mammalian cells

   Depositor

   Sumio Terada

   Article

   Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

   Insert

   anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
   

   Use

   Tags

   cp10GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

   Plasmid

   #79671

   Purpose

   Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLKO.1-CAPRIN1-CDS

   Plasmid

   #136054

   Purpose

   CAPRIN1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CCTCAGCAGAACACTGGATTT)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   CAPRIN1 (CAPRIN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-hSyn-Gαi*-BERKY3

   Plasmid

   #158428

   Purpose

   BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pYPQ133C2.0

    Plasmid

    #99893

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

    Plasmid

    #73475

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-GFP-P2A-N2c(G)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pYPQ131C2.0

    Plasmid

    #99886

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ132C2.0

    Plasmid

    #99889

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-hSyn-Gαq*-BERKY1

    Plasmid

    #158430

    Purpose

    BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. C-BLa 2.0

    Plasmid

    #92060

    Purpose

    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Use

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAP118

    Plasmid

    #105495

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-TAP mLST8 (aka: GbL)

    Plasmid

    #12575

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mLST8 (MLST8 Human)
    

    Use

    Retroviral

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC_mNG2(11)_BSDminus_F0

    Plasmid

    #184162

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-Basic-hCYP26A1P-E4-luciferase

    Plasmid

    #135592

    Purpose

    Short form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vector

    Depositor

    Catharine Ross

    Article

    Zhang et al Gene. 2010 Sep 15;464(1-2):32-43. doi: 1

    Insert

    Short form promoter of human CYP26A1 gene (E4)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Human CYP26A1 promoter (Short Form, E4)

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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