59,541 results

1. pPalmitoyl-mTurquoise2

   Plasmid

   #36209

   Purpose

   In vivo visualization of the membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   p63(1-29) (ARHGEF25 Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-FGFR4-V5/HIS

   Plasmid

   #201109

   Purpose

   expression of human FGFR4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-SMN

   Plasmid

   #37057

   Depositor

   Greg Matera

   Article

   Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

   Insert

   Human SMN1 (SMN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGK-T7/2-CD44v3 (fl)

   Plasmid

   #137814

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v3 (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and N280D in the V3 region

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Venus2-Y705F/S727A-STAT3

   Plasmid

   #123177

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBudORF2-CH

   Plasmid

   #51289

   Purpose

   expresses the codon optimized ORF2 of L1RP with his tag

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

   Insert

   L1 ORF2 (L1RE1 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF1884

   Plasmid

   #143984

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZIC2 (ZIC2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCuo CA Rac1

   Plasmid

   #84643

   Purpose

   constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Constitutively Active, Q61L

   Promoter

   cumate-inducible promoter

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-Cdc42

   Plasmid

   #12201

   Depositor

   Jonathan Chernoff

   Article

   Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2164

    Plasmid

    #142186

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GRHL2 (GRHL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LPAR1-Tango

    Plasmid

    #66418

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    LPAR1 (LPAR1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_HAVCR2_WT

    Plasmid

    #82893

    Purpose

    Gateway Donor vector containing HAVCR2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    HAVCR2 (HAVCR2 Human)
    

    Use

    Gateway entry vector

    Mutation

    R140L

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mApple-Sec61b-C1

    Plasmid

    #90993

    Purpose

    Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mApple-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mApple (red fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEmerald-WASP1-N-14

    Plasmid

    #54315

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    WASP1 (WASF1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCMV 3xFLAG-LC3B WT

    Plasmid

    #123092

    Purpose

    Expresses 3xFLAG-LC3B wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTBNde(min)

    Plasmid

    #15125

    Depositor

    Franco Pagani

    Article

    Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

    Insert

    alpha-globin EDB fibronectin minigene (FN1 Human)
    

    Expression

    Mammalian

    Promoter

    alpha-globin

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

17. p3xFLAG-ATF6

    Plasmid

    #11975

    Purpose

    For mammalian expression of human ATF6 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. YAP1-V5 in pLX304

    Plasmid

    #42555

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-PITCh-ACTB

    Plasmid

    #207748

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of ACTB (ACTB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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