We narrowed to 11,246 results for: SHA

1. pTre2tet)::Fbxl21

   Plasmid

   #51890

   Purpose

   expression of Fbxl21

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   Fbxl21 (Fbxl21 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV tet

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRSM32.2

   Plasmid

   #101635

   Purpose

   Encodes the fluorescent protein Rosmarinus under a CMV promoter.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   Rosmarinus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ShuttleMCP

   Plasmid

   #50959

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   uses portion of the mouse cofilin gene as a mouse…

   Available since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEJS443-pCSDest2-AcrIIC3Nme

   Plasmid

   #85713

   Purpose

   Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Type II-C anti-CRISPR AcrIIC3Nme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS1479-HTP-KanMX6

   Plasmid

   #154085

   Purpose

   For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

   Depositor

   Lidia Vasiljeva

   Article

   Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

   Insert

   his6-TEV-protein A-KanR
   

   Use

   Pcr-based tagging in yeast

   Tags

   his6-TEV-protein A

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSM14

   Plasmid

   #101645

   Purpose

   Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   efasCFP
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.U6gLacZ.hSyn.H2B.RFP

   Plasmid

   #170369

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-Ub-VPS13D_dC

   Plasmid

   #176466

   Purpose

   express VPS13D lacking C-terminal 407 aa in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Use

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   C-terminal 407 aa is deleted.

   Promoter

   CMV

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. kank2_pJet1.2

   Plasmid

   #121980

   Purpose

   for generating kank2 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank2 (LOC571549 Zebrafish)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-myc-mCE(K294A)

    Plasmid

    #82461

    Purpose

    Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mCE (K294A) (Rngtt Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Lysine 294 changed to Alanine

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAST-HDAC4 3A

    Plasmid

    #52978

    Purpose

    insect expression of HDAC4 3A constitutive active mutant

    Depositor

    Biao Wang, Marc Montminy

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    HDAC4 (HDAC4 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    S239,573,748A constitutively active*

    Promoter

    Hsp70

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Clover-ZO1-C-14

    Plasmid

    #56375

    Purpose

    Localization: Tight Junctions, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    ZO1 (TJP1 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. PDZK1_PDZ_1

    Plasmid

    #103927

    Purpose

    Protein expression and purification of PDZK1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SLC9A3R2_PDZ_2

    Plasmid

    #103926

    Purpose

    Protein expression and purification of NHERF2 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PDZK1_PDZ_4

    Plasmid

    #103945

    Purpose

    Protein expression and purification of PDZK1 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLN481 (FuGW-G8p-IL12-1Pe)

    Plasmid

    #105191

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-IL12-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS-DTA miR-106b~25

    Plasmid

    #19030

    Depositor

    Tyler Jacks

    Article

    Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

    Insert

    microRNA 106a-25 (Mir25 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. SMT205

    Plasmid

    #134817

    Purpose

    SMT201 with mutated RBS for DHFR, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDB368

    Plasmid

    #110276

    Purpose

    gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Insert

    gHH_44
    

    Use

    Tags

    10-His, Tev recognition peptide, and mature DsbC …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS430-pCSDest2-AcrIIC1Boe

    Plasmid

    #85678

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC1Boe
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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