19,654 results

1. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Promoter

   pRSV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. FKBP-V5-AP-nes_pLX304

   Plasmid

   #120912

   Purpose

   Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   FKBP-V5-AP-NES
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMS-II

   Plasmid

   #204765

   Purpose

   GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC7-20XUAS-IVS-mCD8::GFP

   Plasmid

   #26220

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   mCD8::GFP
   

   Expression

   Insect

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3b-8XGliBS:EGFP

   Plasmid

   #84602

   Purpose

   Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

   Depositor

   James Chen

   Article

   Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   8X Gli-binding site

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ornithine transcarbamylase (OTC)

   Plasmid

   #71877

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC (Otc Rat)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Syn-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #125693

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pAG416GAL-ccdB

    Plasmid

    #14147

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2C-EGFP

    Plasmid

    #69071

    Purpose

    Expresses EGFP under MERVL_LTR

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA-mTmG

    Plasmid

    #26123

    Depositor

    Liqun Luo

    Insert

    Precursor mT/mG
    

    Tags

    membrane tag

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCB-hDot1Lmut

    Plasmid

    #74174

    Purpose

    For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    hDot1L 163 GSG to RCR mutation*

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pMJH46

    Plasmid

    #67272

    Purpose

    Conjugally transferable red recombinogenic plasmid

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    traJ, cat, oriT
    

    Use

    Red recombinase

    Promoter

    cat gene promoter from PGNS-BAC

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163055

    Purpose

    Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-myc-Cdc42-T17N

    Plasmid

    #12973

    Depositor

    Gary Bokoch

    Insert

    Cdc42 DN (CDC42 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hs.KRAS4b G12D

    Plasmid

    #83131

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12D

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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