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Showing: 5921 - 5940 of 65351 results

  5. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Publication
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  8. Str-KDEL_ManII-SBP-EGFP

    Plasmid
    #65252
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    65252-plasmid-map-sequence-id-120582

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  10. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    62204-plasmid-map-sequence-id-101817

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Showing: 5921 - 5940 of 65351 results