We narrowed to 11,743 results for: pcdna

1. YFP(1-158)-beta-5 in pcDNAI/Amp

   Plasmid

   #54470

   Purpose

   An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
   

   Use

   Tags

   YFP(1-158) was fused to the amino terminus of Gbe…

   Expression

   Mammalian

   Mutation

   YFP (1-158) includes a substitution of Met for Gl…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

   Plasmid

   #167313

   Purpose

   Template pDNA to prepare DD-CaVT mRNA

   Depositor

   Hirohide Saito

   Article

   Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

   Insert

   DD-CaVT
   

   Use

   Synthetic Biology

   Tags

   DYK-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-myc3-CUL4A delta N100

   Plasmid

   #19953

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CUL4A (cullin 4A) (CUL4A Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   The longest published clone (Q13619)

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-FLAG-NEMO-C54/347A

   Plasmid

   #27268

   Depositor

   Thomas Gilmore

   Article

   Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Cys54Ala Cys347Ala

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3/flag-pBirA(1-G78)-FRB

   Plasmid

   #154447

   Purpose

   expresses pBirA(1-G78)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-G78)-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-FLAG-Dicer-5'-pocket mutant

   Plasmid

   #51368

   Depositor

   Narry Kim

   Article

   Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   5' pocket mutant (R778A, R780A, R811A, H982A…

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Venus-Venus-FLARE-AKAR

   Plasmid

   #123331

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-AKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HA-REDD1 R133A/P139A (#626)

   Plasmid

   #99946

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R133A/P139A

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

   Plasmid

   #16754

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-trunc before PDZ (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncated before PDZ

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA1 3XFlag ATXN1[15Q]-delAXH

    Plasmid

    #33241

    Depositor

    Huda Zoghbi

    Article

    Lam et al Cell. 2006 Dec 29;127(7):1335-47.

    Insert

    ATXN1[2Q]-delAXH (ATXN1 Human)
    

    Use

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

    Plasmid

    #123342

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 myc-mβTrCP F-box (1-247)

    Plasmid

    #62976

    Purpose

    Expresses myc-tagged mouse βTrCP residues 1-247

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    3x myc

    Expression

    Mammalian

    Mutation

    codes for residues 1-247

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid

    #157693

    Purpose

    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. XE253 pCDNA3.1(zeo)-hDsh3-82-716

    Plasmid

    #16756

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-82-716-Dsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa 82-716

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-FLAG-SBP-STAG2 (S97X)

    Plasmid

    #73964

    Purpose

    STAG2 cDNA (CCDS43990) S97X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S97X

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 Flag MFF iso5 S172A

    Plasmid

    #74382

    Purpose

    Flag tagged human S172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3-IgL-C99-T4L-rTA

    Plasmid

    #177840

    Purpose

    Tango assay

    Depositor

    Karsten Melcher

    Article

    Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

    Insert

    C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

    Plasmid

    #172581

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 Flag MFF iso5 S155,172A

    Plasmid

    #74383

    Purpose

    Flag tagged human S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    See 155 Ala, Ser 172 Ala (numbering based on MFF …

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only