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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,700 results for: SHE

Showing: 8911 - 8940 of 17700 results

  2. CD44Z-c011

    Plasmid
    #175455
    Purpose
    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175455-plasmid-map-sequence-id-353216

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  4. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110420-plasmid-map-sequence-id-213695

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  5. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  6. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

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  10. Hs.KRAS4b G12R

    Plasmid
    #83143
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12R
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83143-plasmid-map-sequence-id-158106

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  11. pTP-AAV-ChATen-mCherry

    Plasmid
    #193741
    Purpose
    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.
    Depositor
    Hynek Wichterle
    Insert
    ChAT enhancer, mini promoter, synthetic intron, mCherry
    Use
    AAV and Mouse Targeting
    Expression
    Mammalian
    Promoter
    1000 bp ChAT enhancer with a 32 bp minimal promot…
    Available Since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    193741-plasmid-map-sequence-id-450307

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  12. pBabe-Kras G12V-T35S

    Plasmid
    #58900
    Purpose
    Expresses human Kras with G12V and T35S mutations
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G12V and T35S mutations (effector binding mutant)
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  16. Hs.KRAS4b S17N

    Plasmid
    #83156
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    S17N
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83156-plasmid-map-sequence-id-158119

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  22. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Promoter
    pdx
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

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  23. Hs.KRAS4b Q61L

    Plasmid
    #83134
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61L
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83134-plasmid-map-sequence-id-158097

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  25. C1QBP

    Plasmid
    #32863
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C1QBP (C1QBP Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids E89-Q282 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8911 - 8940 of 17700 results