We narrowed to 9,006 results for: Pol

1. Cas9_P2A_T4pol

   Plasmid

   #190146

   Purpose

   Expresses SpCas9 and T4pol

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_T4pol
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9_P2A_POLB

   Plasmid

   #190145

   Purpose

   Expresses SpCas9 and POLB

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_POLB
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPOLY_new

   Plasmid

   #179276

   Purpose

   Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLz58_polyPro_CsoS2_CTP

   Plasmid

   #140850

   Purpose

   Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 C-terminal peptide
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz55_polyPro_random_Npeptide

   Plasmid

   #140846

   Purpose

   Same as pLz47 but with randomized N*-peptide sequence

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with randomized CsoS2 N-peptide
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz56_polyPro_CsoS2_MR_r1

   Plasmid

   #140848

   Purpose

   Same as pLz47 but with MR repeat 1 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 MR repeat 1
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLz47_polyPro_CsoS2_Npeptide

   Plasmid

   #140845

   Purpose

   Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 N-peptide
   

   Use

   Tags

   Hexahistidine tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLz57_polyPro_CsoS2_CTD_r8

   Plasmid

   #140849

   Purpose

   Same as pLz47 but with CTD repeat 8 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 CTD repeat 8
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PoliIFN-γ

   Plasmid

   #107289

   Purpose

   Plasmid can be used for secretion of fish Interferon gamma

   Depositor

   Bohdan Schneider

   Article

   Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

   Insert

   Interferon gamma
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. gag-P3-pol

    Plasmid

    #211374

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-P3-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. HO-Poly-KanMX4-HO

    Plasmid

    #51662

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. HDR-HaloPOLQ

    Plasmid

    #211637

    Purpose

    halo donor

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    Puro-LoxHaloTag
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-hPolB

    Plasmid

    #70761

    Purpose

    bacterial expression of human DNA Polymerase beta (POLB)

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    POLB (POLB Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    R333L

    Promoter

    T5/lac operon

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-GBDpol

    Plasmid

    #165501

    Purpose

    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Pyrococcus Sp. Heat-Stable DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCas9-polyA

    Plasmid

    #72602

    Purpose

    express hCas9 byT7 promoter with polyadenine tails

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

    Insert

    Cas9-polyadenine
    

    Use

    Tags

    81-bp polyadenylation

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pOpen-SP6rpol

    Plasmid

    #165516

    Purpose

    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    SP6 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pBluescript II SK--POLQ

    Plasmid

    #64873

    Purpose

    cloning vector for human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMAH-POLY

    Plasmid

    #64915

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

    Inserts

    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOpen-KTHEAQpol

    Plasmid

    #165542

    Purpose

    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    KlenTaq1 polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pEP PolI

    Plasmid

    #11722

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I EP (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D424A, I709N, A759R. Error-prone version of PolI.

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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26. pOpen-Pwopol

    Plasmid

    #165562

    Purpose

    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Pwo DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pWT PolI

    Plasmid

    #11721

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pOpen-K12polLF

    Plasmid

    #165511

    Purpose

    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. mCherry-POLE1

    Plasmid

    #215140

    Purpose

    Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    POLE1 (POLE Human)
    

    Use

    Retroviral

    Tags

    mCherry_FWR

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acid 80-86 and 211-217 …

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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