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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,443 results for: 129

Showing: 101 - 150 of 5443 results

  3. pRK5-myc-RhoA-T19N

    Plasmid
    #12963
    Depositor
    Gary Bokoch
    Insert
    RhoA DN (RHOA Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T19N Dominant negative
    Promoter
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    12963-plasmid-map-sequence-id-223086

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  5. pGEX-2T-Rac1-Q61L

    Plasmid
    #12979
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  10. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129761-plasmid-map-sequence-id-422377

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  17. pRK5-myc-Cdc42-Q61L

    Plasmid
    #12974
    Depositor
    Gary Bokoch
    Insert
    Cdc42 constitutively active (CDC42 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    12974-plasmid-map-sequence-id-132746

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  18. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid
    #129383
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP
    Depositor
    Silvestro Conticello
    Article
    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:
    Insert
    mCherry-ApoB-Tag-BFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    129383-plasmid-map-sequence-id-254328

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  19. pTfR-mNeonGreen

    Plasmid
    #129608
    Purpose
    In vivo visualization of Transferrin receptors (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Transferrin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    129608-plasmid-map-sequence-id-261583

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  20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129027-plasmid-map-sequence-id-256129

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  22. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

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  23. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid
    #129594
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    129594-plasmid-map-sequence-id-261157

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  30. pRK5-myc-Cdc42-T17N

    Plasmid
    #12973
    Depositor
    Gary Bokoch
    Insert
    Cdc42 DN (CDC42 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T17N Dominant negative
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12973-plasmid-map-sequence-id-134936

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  31. pCDH-UBC-DSRED-LUC-EF1 Hygro

    Plasmid
    #129437
    Purpose
    express dsred-luc chimera
    Depositor
    Oskar Laur
    Insert
    dsred-luc
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129437-plasmid-map-sequence-id-256215

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  32. mNeongreen-PXN

    Plasmid
    #129604
    Purpose
    In vivo visualization of focal adhesions (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Paxillin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    129604-plasmid-map-sequence-id-260227

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  36. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid
    #129628
    Purpose
    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes
    Depositor
    Dorus Gadella
    Article
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Insert
    FHL-cpmTq2-Calcium-lifetime-sensor
    Use
    Tags
    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV,&Rhamnose
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    129628-plasmid-map-sequence-id-260204

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  38. pGEX-4T3-RhoA-Q63L

    Plasmid
    #12961
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q63L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  40. pGEM2 hTSP-1

    Plasmid
    #12993
    Depositor
    Jack Lawler
    Insert
    TSP-1 (THBS1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 28, 2006
    Availability
    Academic Institutions and Nonprofits only
    12993-plasmid-map-sequence-id-234345

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  43. pGEX-2T-RhoA-wt

    Plasmid
    #12959
    Depositor
    Gary Bokoch
    Insert
    RhoA (RHOA Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 150 of 5443 results