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Showing: 101 - 150 of 13385 results

  5. pUt-mNF-BACH1

    Plasmid
    #199219
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199219-plasmid-map-sequence-id-396467

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  8. pAAV.hSynap.iAChSnFR-Venus-NULL

    Plasmid
    #137953
    Purpose
    Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (yellow version, non-binding variant)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    cpSFGFP replaced with cpSFVenus; Y140A binding si…
    Promoter
    hSynapsin
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137953-plasmid-map-sequence-id-271684

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  9. pOZ-FH-C Bach1 S990A

    Plasmid
    #73647
    Purpose
    Retroviral expression of Bach1 S990A mutant (BRCA binding mutant).
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Retroviral
    Tags
    FLAG and His
    Expression
    Mammalian
    Mutation
    S990A (BRCA binding mutant)
    Promoter
    unknown
    Available Since
    April 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    73647-plasmid-map-sequence-id-240738

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  10. pOZ-FH-C Bach1 K52R

    Plasmid
    #73645
    Purpose
    Retroviral expression of Bach1 K52Rmutant (helicase null)
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Retroviral
    Tags
    FLAG and His
    Expression
    Mammalian
    Mutation
    K52R (helicase null)
    Promoter
    unknown
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only

  25. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  27. gWiz_BM40-vAchT-Cytosolic-VL-hIgG1-CL

    Plasmid
    #218447
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    218447-plasmid-map-sequence-id-431100

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  28. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pBS-AMVpl-δWT-nAchR

    Plasmid
    #97188
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    97188-plasmid-map-sequence-id-191155

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  30. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pBS-AMVpl-γWT-nAchR

    Plasmid
    #97187
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97187-plasmid-map-sequence-id-183032

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  32. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pBS-AMVpl-β1WT-nAchR

    Plasmid
    #97080
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97080-plasmid-map-sequence-id-189664

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  35. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

    Plasmid
    #137960
    Purpose
    Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding yellow variant)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    Y140A binding site mutation
    Promoter
    CAG
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137960-plasmid-map-sequence-id-271575

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  41. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. Ec holA-pET(11a)(ACH-)

    Plasmid
    #237233
    Purpose
    over-express E. coli holA (Pol III delta subunit)
    Depositor
    Michael O'Donnell
    Insert
    holA (delta) (holA E. coli)
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237233-plasmid-map-sequence-id-469254

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  50. Ec holB-pET(11a)(ACH-)

    Plasmid
    #237234
    Purpose
    over-express E. coli holB (Pol III delta prime subunit)
    Depositor
    Michael O'Donnell
    Insert
    holB (delta prime) (holB E. coli)
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237234-plasmid-map-sequence-id-469255

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Showing: 101 - 150 of 13385 results