We narrowed to 2,461 results for: T2A

1. pCAGGS-mTagBFP2-T2A-iCre-ERT2

   Plasmid

   #128168

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CAG-IntCCCre-T2A-CopGFP

   Plasmid

   #160501

   Purpose

   Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   IntCCCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   CopGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-pDisplay-Halo-T2A-EGFP

   Plasmid

   #223498

   Purpose

   Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

   Depositor

   James Frank

   Insert

   HaloTag and EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAND1-T2A-dTom-PGK-Neo

   Plasmid

   #209838

   Purpose

   Tomato reporter plasmid targeting the human HAND1 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

   Insert

   HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
   

   Use

   Human targeting

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-T2A-Gal4-3xP3-RFP

   Plasmid

   #127556

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE-UBC-mCD55-T2A-GFP

   Plasmid

   #205450

   Purpose

   lentiviral plasmid for expression of mouse CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD55 (Cd55 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMT-PT2A-GFP-Tdtomato-iRFP670

   Plasmid

   #111812

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   Tdtomato
   iRFP670
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Puro-T2A-mCitrine-hSmad2-donor

    Plasmid

    #203663

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    Smad2
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Has Service

    AAV8

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid

    #223503

    Purpose

    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

    Depositor

    James Frank

    Insert

    SNAP-tag and EGFP
    

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVTR(T7A)-Cas9-T2A-mClover3

    Plasmid

    #172293

    Purpose

    In vitro transcription of Cas9-T2A-mClover3 synthetic construct

    Depositor

    Patrik Verstreken

    Insert

    Cas9-T2A-mClover3
    

    Use

    CRISPR

    Promoter

    T7 promoter A-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

    Plasmid

    #62951

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-UBC-H2-Kb SCT-T2A-GFP

    Plasmid

    #205454

    Purpose

    lentiviral plasmid for expression of mouse H2-Kb SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    H2-Kb SCT (H2-K1 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-MSCV-4xS1m-GFP-T2A-PU

    Plasmid

    #138581

    Purpose

    For in vivo tagging

    Depositor

    Yin-Yuan Mo

    Article

    Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    S1m tag

    Promoter

    MSCV

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV-IFP2.0 T2A HO1

    Plasmid

    #59427

    Purpose

    Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

    Depositor

    Xiaokun Shu

    Article

    Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

    Insert

    IFP2.0T2AHO1 IRES EGFP
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVTR(T7A)-PuroR-T2A-mScarlet

    Plasmid

    #172301

    Purpose

    In vitro transcription of PuroR-T2A-mScarlet synthetic construct

    Depositor

    Patrik Verstreken

    Insert

    PuroR-T2A-mScarlet
    

    Use

    Other

    Promoter

    T7 promoter A-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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