We narrowed to 14,011 results for: flag

1. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM60-Auf1

   Plasmid

   #38242

   Purpose

   3rd generation lentiviral vector

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   AUF1 (HNRNPD Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR023_PIE-PUM2

   Plasmid

   #204819

   Purpose

   Expression PIE-PUM2 fusion protein in mammalian cells

   Depositor

   Xiaochang Zhang

   Article

   Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

   Insert

   Pumilio 2 (PUM2 Human)
   

   Use

   Tags

   HA-Flag-rApobec1 and huADAR2dd

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-NLyn-Venus-Zdk1

   Plasmid

   #81042

   Purpose

   mammalian expression of plasma-membrane anchored Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Use

   Tags

   6x His tag, FLAG, NLyn, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn-IRES2-GFP

   Plasmid

   #234875

   Purpose

   Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSYn

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MIG(F)-CRel

   Plasmid

   #26984

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Insert

   CRel (Rel Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pBEL2108

   Plasmid

   #201969

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

   Insert

   attB-3C-eGFP-4xGly-TEV-Flag-6xHis
   

   Use

   Orbit integrating plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRG706_lexO-SbfI_LexA-TF_LEU2MX

   Plasmid

   #154818

   Purpose

   Inducible expression of SbfI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   SbfI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LNCX TbetaRII DN

   Plasmid

   #12640

   Depositor

   Rik Derynck

   Article

   Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

   Insert

   TGF-beta receptor II (TGFBR2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: truncated cytoplasmically

   Promoter

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCKPC4

    Plasmid

    #31366

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pBiex-1 Myosin VI HMM_eGFP

    Plasmid

    #82714

    Purpose

    Expresses Myosin VI HMM with a C-terminal eGFP.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Myosin VI
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRAIP-SFB

    Plasmid

    #101769

    Purpose

    Mammalian expression of TRAIP fusion protein

    Depositor

    Michael Huen

    Article

    Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

    Insert

    TRAIP / RNF206 (TRAIP Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRG646_lexO-HO_LexA-TF_LEU2MX

    Plasmid

    #154815

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-LplA(W37V) Coumarin PRIME ligase

    Plasmid

    #25845

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37V
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37V

    Promoter

    Available Since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRG659_lexO-FseI_LexA-TF_LEU2MX

    Plasmid

    #154816

    Purpose

    Inducible expression of FseI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    FseI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLM802

    Plasmid

    #27203

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMLM800

    Plasmid

    #27202

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDest490-OPN-b

    Plasmid

    #17591

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-b (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 1–175, 217–942; See Depositor Comments

    Promoter

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST-Ki

    Plasmid

    #44563

    Purpose

    for integrative constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-PP7v3

    Plasmid

    #107230

    Purpose

    Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    24xPP7v3
    

    Use

    Tags

    3x FLAG-tag and P2A site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-tag2B-PISP/AIPP1

    Plasmid

    #47606

    Depositor

    Emanuel Strehler

    Article

    Goellner et al Ann N Y Acad Sci. 2003 Apr;986:461-71.

    Insert

    PISP (PDZD11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

22. DT035A

    Plasmid

    #159397

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tac-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3 tdAPX W41F

    Plasmid

    #40863

    Purpose

    Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    tandem dimer pea APX W41F
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    W41F mutation

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJO629

    Plasmid

    #104342

    Purpose

    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

    Insert

    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. POLN_pDEST17

    Plasmid

    #64885

    Purpose

    bacterial expression of POLN for purification

    Depositor

    Richard Wood

    Article

    Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

    Insert

    POLN (POLN Human)
    

    Use

    Tags

    Flag and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ciCas9_pcDNA5

    Plasmid

    #100550

    Purpose

    Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLDPuro-MmnSR100N

    Plasmid

    #35171

    Depositor

    Benjamin Blencowe

    Article

    Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

    Insert

    Srrm4 (Srrm4 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRG714_lexO-AscI_LexA-TF_URA3MX

    Plasmid

    #154822

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTriEx-NTOM20-mVenus-Zdk3

    Plasmid

    #81012

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk3
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRG645_lexO-AscI_LexA-TF_LEU2MX

    Plasmid

    #154813

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge