We narrowed to 7,184 results for: RAP

1. FCM101

   Plasmid

   #194298

   Purpose

   E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

   Depositor

   Shohei Koide

   Article

   Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

   Insert

   FCM101
   

   Use

   Tags

   His tag, FLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-4F2-mCherry

   Plasmid

   #73421

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 4F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P4F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. KAT2A sgRNA2

   Plasmid

   #138186

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT2A (KAT2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. KAT2A sgRNA1

   Plasmid

   #138185

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT2A (KAT2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLC-BFP-FADD

   Plasmid

   #75166

   Purpose

   LentiCRISPR-BFP with sgRNA targeting human FADD

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   FADD sgRNA (FADD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-TagRFP658-P2A-GFP

   Plasmid

   #178971

   Purpose

   Expresses TagRFP658 and GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   TagRFP658-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-extGluc-NGFR

   Plasmid

   #104832

   Purpose

   extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   extGLuc
   NGFR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRosa26-EF1a-hCas9IRESneo

   Plasmid

   #67987

   Purpose

   Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-hCas9IRESneopA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-ARF-mOrange2

   Plasmid

   #110731

   Purpose

   Mammalian Expression of p14ARF IRES H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   p14ARF IRES H2B-mOrange2 (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

    Plasmid

    #216323

    Purpose

    Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cas9-VPR + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV 3xFLAG-ATG3 C264A

    Plasmid

    #129297

    Purpose

    Expresses 3xFLAG-ATG3 C264A in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Cysteine 264 to Alanine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL40C_PGKintron_Cas9_Green

    Plasmid

    #134966

    Purpose

    Lentiviral vector coding Cas9 and mNeonGreen

    Depositor

    Beat Bornhauser

    Article

    Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

    Insert

    Cas9-P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK promoter with beta-globin intron

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pExp-RBD-CHis

    Plasmid

    #195002

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

    Plasmid

    #171963

    Purpose

    Donor plasmid for CLTX T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a-tm CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. VBS mutant of alpha catenin sensor

    Plasmid

    #71710

    Purpose

    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin ΔVBS FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 316-405 in α -catenin…

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER20 MEF2C S222D

    Plasmid

    #107234

    Purpose

    MEF2C phosphomimetic mutant serine to aspartic acid

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPR_BRD003 sgKEAP1-1

    Plasmid

    #74985

    Purpose

    sgKEAP1-1, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PLL5.0-Anillin ShRNA-GFP-AnillinFL

    Plasmid

    #187272

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6, CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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