We narrowed to 54,981 results for: ran

1. pKDR-EF1a.1

   Plasmid

   #183568

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 1
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDR-CBh.21

   Plasmid

   #183565

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 21
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

   Plasmid

   #182376

   Purpose

   Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Inserts

   3xFlag
   mito-mTFP1
   

   Expression

   Mammalian

   Promoter

   CMV promoter and SV40 promoter

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPQD0CM0058

   Plasmid

   #177022

   Purpose

   Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6
   

   Use

   Synthetic Biology

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPQD0CM0059

   Plasmid

   #177023

   Purpose

   Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
   

   Use

   Synthetic Biology

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPQD0CM0063

   Plasmid

   #177021

   Purpose

   Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geraniol synthase
   

   Use

   Synthetic Biology

   Mutation

   transit peptide removed

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJo55

   Plasmid

   #159623

   Purpose

   An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

   Depositor

   Vasiliy Taranov

   Article

   Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-HA-PGRMC1-1-54

   Plasmid

   #173472

   Purpose

   PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1 (PGRMC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted for amino acids 55-194

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-HA-PGRMC1-21-195

   Plasmid

   #173473

   Purpose

   PGRMC1 lacking the N-terminal region upstream of the transmembrane region

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1 (PGRMC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted for amino acids 1-20

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAraQCas-BsaI

    Plasmid

    #170633

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEL2082

    Plasmid

    #155162

    Purpose

    Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    (MlaFΔ[248-269])-(6xHis-TEV-MlaB)
    

    Expression

    Bacterial

    Mutation

    deleted amino acids 248-269 in MlaF

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBEL1514

    Plasmid

    #155147

    Purpose

    Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
    

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEL1965

    Plasmid

    #155152

    Purpose

    Expresses MlaF(1-246)-(6 aa linker)-GCN4

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEL1966

    Plasmid

    #155153

    Purpose

    Expresses MlaF(1-246)-(10 aa linker)-GCN4

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL1967

    Plasmid

    #155154

    Purpose

    Expresses MlaF(1-246)-(14 aa linker)-GCN4

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGST-POLA1-N0

    Plasmid

    #160983

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGST-POLA1-N1-2A

    Plasmid

    #160986

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W84A/D88A

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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