We narrowed to 29,795 results for: REP

1. LLhA-pHG165a

   Plasmid

   #90044

   Purpose

   Constitutive expression of a chimeric LacI:AscG transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLhE-pHG165

   Plasmid

   #90045

   Purpose

   Constitutive expression of a chimeric LacI:CelR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLhC-pHG165a

   Plasmid

   #90046

   Purpose

   Constitutive expression of a chimeric LacI:CytR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-CAG-mNanogHD-pgk-hph

   Plasmid

   #74909

   Purpose

   Mammalian expression of mNanog Homeodomain

   Depositor

   Jose Silva

   Article

   Theunissen et al Development. 2011 Nov;138(22):4853-65. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJC25R1+4stop

   Plasmid

   #17723

   Depositor

   Susan Lindquist

   Article

   Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

   Insert

   Sup35R1+4 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E.coli expression plasmid for NM-region of Sup35 …

   Promoter

   Available Since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiPGK Hygro DEST ERKKTRmCerulean3

   Plasmid

   #90229

   Purpose

   Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMitoTimer

   Plasmid

   #52659

   Purpose

   Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

   Depositor

   Zhen Yan

   Article

   Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

   Insert

   DsRed1-E5
   

   Use

   Dsred

   Tags

   human cytochrome c oxidase subunit VIII mitochond…

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti F9-TetR-EGFP-IRES-PuroR

   Plasmid

   #117049

   Purpose

   lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Insert

   F9-TetR-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   F9 promoter

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. NL4-3Δ6-drEGFP-IRES-mThy1.2

   Plasmid

   #207881

   Purpose

   HIV reporter vector expressing dGFP and mThy1.2 from Env locus

   Depositor

   Edward Browne

   Article

   Peterson et al Cell Chem Biol. 2023 Dec 21;30(12):1617-

   Insert

   destabilized EGFP-IRES-mThy1.2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   5' lentiviral LTR

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-ZIKVA-GFP-puro

    Plasmid

    #140090

    Purpose

    Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    ZIKVA-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-DENV2A-GFP-puro

    Plasmid

    #140089

    Purpose

    Fluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    DENV2A-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

    Plasmid

    #213826

    Purpose

    Expresses human PRDM6 with a C-terminal V5 epitope tag.

    Depositor

    Bjoern Schwer

    Article

    Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

    Insert

    PRDM6 (PRDM6 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. XRE-pNL1.3[secNLuc]

    Plasmid

    #182295

    Purpose

    Secreted Luciferase reporter for AhR activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    Xenobiotic Response Element (CYP1A1 Human)
    

    Use

    Tags

    secNluc

    Expression

    Mammalian

    Mutation

    Promoter

    XRE

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUW-tetO-Pu.1

    Plasmid

    #139839

    Purpose

    doxycycline-inducible expression of mouse Pu.1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Pu.1 (Spi1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-LUC2CP/ARE

    Plasmid

    #62857

    Purpose

    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    destabilizing sequences (DS) added to the C termi…

    Promoter

    CMV

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmVen-RPA70-IRES-RPA32-IRES-RPA14

    Plasmid

    #211431

    Purpose

    mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Use

    Tags

    mVenus (on RPA70)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pKrox24(2xD-E)DsRed

    Plasmid

    #200111

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG397

    Plasmid

    #226733

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    OMP25 (SYNJ2BP Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRPA70-IRES-RPA32-IRES-RPA14

    Plasmid

    #211432

    Purpose

    mammalian expression of human RPA70 and RPA32 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 NL

    Plasmid

    #113442

    Purpose

    NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPBbsr-AcGFP1-ORP9-PH

    Plasmid

    #214270

    Purpose

    Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    AcGFP1-ORP9(1-111) (Osbpl9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. Oct4-IRES-eGFP-PGK-Neo

    Plasmid

    #48681

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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