We narrowed to 17,700 results for: SHE

1. huntingtin_V2095-V3138_CbioCflag

   Plasmid

   #194764

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV and P10

   Available Since

   July 27, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. huntingtin_T97-M2069_NbioCflag

   Plasmid

   #194763

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV and P10

   Available Since

   July 26, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MAP3K3

   Plasmid

   #38937

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAP3K3 (MAPK3 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221_SMAD2_HAe2_Bsd

   Plasmid

   #167166

   Purpose

   Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

   Depositor

   Alexander Loewer

   Insert

   Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
   

   Use

   Repair template for gene editing in human cells

   Tags

   blasticidin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSM42

   Plasmid

   #113942

   Purpose

   Constitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1a

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   jRGECO1a
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SDC229-B03

   Plasmid

   #58763

   Purpose

   bacterial expression of PDB 4PYZ

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4PYZ (USP7 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   Aug. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On-HDAC1-shRNA3

   Plasmid

   #90243

   Purpose

   Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM_noro_S2_lacZA

   Plasmid

   #118816

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S2
   

   Expression

   Bacterial

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GWF135

    Plasmid

    #133614

    Purpose

    ANR-ANR-BFP-CAAX

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    ANR-ANR-BFP-CAAX
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ataxin3:1-431_Q79_BV

    Plasmid

    #209733

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    6xHis-SBP-TEV

    Expression

    Bacterial

    Promoter

    Polyhedrin

    Available Since

    Nov. 13, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCR4-MBII52-probe

    Plasmid

    #67647

    Purpose

    antisense RNA probe for RNase protection or nothern

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII52 snoRNA (Snord115 Mouse)
    

    Use

    For in vitro transcription

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pSA107

    Plasmid

    #59785

    Purpose

    drives ZIF-1 protein in C. elegans ubiquitously

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    cdc-42 promoter (NEWENTRY Nematode)
    zif-1 coding sequence (zif-1 )
    unc-54 3' UTR
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPTN1-55-EGFP

    Plasmid

    #68838

    Purpose

    deleted OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-55 only

    Promoter

    CMV

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. AKT3

    Plasmid

    #39038

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKT3 (AKT3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. MS2_SC2_S_Omicron

    Plasmid

    #180229

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Omicron (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. MS2_SC2_S_WT

    Plasmid

    #175044

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    WT (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDM_noro_S6_lacZA

    Plasmid

    #118817

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S6
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSP64T-dnWnt-11

    Plasmid

    #16857

    Depositor

    Jim Smith

    Article

    Tada et al Development. 2000 May . 127(10):2227-38.

    Insert

    dnWnt-11 (wnt11b.L Frog)
    

    Use

    Xenopus expression

    Mutation

    amino acids 1-282. Dominant Negative.

    Available Since

    May 27, 2008

    Availability

    Academic Institutions and Nonprofits only

20. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)MBII85

    Plasmid

    #67643

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

    Plasmid

    #39300

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S16A, R17A, H18A, D19A, W23A and N25A

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pCDNA3-FLAG-SENP3-C526A

    Plasmid

    #115522

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

    Insert

    SENP3-C526A (Senp3 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C526A

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLJM1-FLAG-Sestrin2-E451A

    Plasmid

    #73677

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

25. CYP7A1 (3SN5)

    Plasmid

    #32874

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP7A1 (CYP7A1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 25-503 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pTorPE-Y-GECO2f

    Plasmid

    #105065

    Purpose

    A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2f
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5-FLAG-Sestrin2-S190W

    Plasmid

    #73670

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S190W changed serine 190 to tryptophan

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

28. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA6-rbNHERF1

    Plasmid

    #32942

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLC9A3R1 (SLC9A3R1 rabbit)
    

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUASp YFP Rac1 CA

    Plasmid

    #52235

    Purpose

    Drosophila expression of YFP-Rac1 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only