We narrowed to 9,951 results for: Uty

1. pTwist-SARS-CoV-2 Spike-deltaC18 Danish Mink deltaFVI

   Plasmid

   #212372

   Purpose

   Encodes SARS-CoV-2 spike deltaH69-H70, Y453F, D614G, I692V, M1229I for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike deltaH69-H70, Y453F, D614G, I692V, M1229I (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   deltaH69-H70, Y453F, D614G, I692V, M1229I

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Spike-deltaC18 deltaL242-L244+R246I (ATA)

   Plasmid

   #212357

   Purpose

   Encodes SARS-CoV-2 spike deltaL242-L244, R246I for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike deltaL242-L244, R246I (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   deltaL242-L244, R246I

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Spike-deltaC18 SAK005321 (Wrong L242H)

   Plasmid

   #212350

   Purpose

   Encodes SARS-CoV-2 spike D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

   Plasmid

   #223162

   Purpose

   Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

   Plasmid

   #223143

   Purpose

   Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 111-173

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.23-hs737

   Plasmid

   #222472

   Purpose

   Luciferase vector containing the hs737 enhancer sequence (reference sequence).

   Depositor

   Tychele Turner

   Article

   Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

   Insert

   hs737 enhancer (reference sequence) (LOC110120928 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

   Plasmid

   #223153

   Purpose

   Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 111-173

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

   Plasmid

   #223161

   Purpose

   Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

   Plasmid

   #223166

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid

    #169835

    Purpose

    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Substitution of amino acids F1308 - C1455 with a …

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCRbluntII-pHES7-STOPgRNA

    Plasmid

    #204349

    Purpose

    gRNA to target pig HES7 stop codon

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
    

    Expression

    Mammalian

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

    Plasmid

    #185701

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(FCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

    Plasmid

    #185705

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PDCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

    Plasmid

    #185703

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(SCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

    Plasmid

    #185704

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

    Plasmid

    #185702

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(BCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEXSISERS_NLuc_FRT-PuroR

    Plasmid

    #177865

    Purpose

    Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    NLuc-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Tags

    OLLAS

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEXSISERS_BSD_FRT-PuroR

    Plasmid

    #177867

    Purpose

    Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    BSD-based EXSISERS
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    OLLAS

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEXSISERS_SurfaceHaloTag_FRT-PuroR

    Plasmid

    #177869

    Purpose

    Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    Surface-HaloTag-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Cstem-Tac(5A)

    Plasmid

    #162497

    Purpose

    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five threonine residues are mutated into alan…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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