159,557 results

1. pAAV-CBh-Alb-mNG

   Plasmid

   #183466

   Purpose

   To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH_blast_MCS_Nard_GFP_LAMIN

   Plasmid

   #167340

   Purpose

   Expresses GFP fused to lamin A (LA) in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_HUWE1_3xFLAG

   Plasmid

   #187155

   Purpose

   mammalian cell expression of HUWE1-3xFLAG

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. iTol2Amp-γ-crystallin:RFP

   Plasmid

   #108455

   Purpose

   This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

   Insert

   Tol2-Amp-y-cryst-mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2538

   Plasmid

   #144036

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX4 (SOX4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-p3-PE(1153-dRnH)-C-term

   Plasmid

   #187182

   Purpose

   p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

   Depositor

   Gerald Schwank

   Article

   Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

   Insert

   PE2 from plasmid #132775 with shortened RnaseH domain
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-ING3

   Plasmid

   #79070

   Purpose

   Expression of p47 ING3 in mammalian cells

   Depositor

   Karl Riabowol

   Insert

   ING3 (p47 ING3 isoform) (ING3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-hHSF1 WT

   Plasmid

   #71724

   Purpose

   plasmid expressing myc-his tag hHSF1 WT

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Tags

   MYC 6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. NES-EGFP-cPHx3

   Plasmid

   #116855

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amino acids 169-329 (tandem trimer)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ

    Plasmid

    #206981

    Purpose

    empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLY095-EFS-ERBB2 HER2-Blast-WPRE

    Plasmid

    #192196

    Purpose

    Lenti-HER2-OE-Blast

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-HER2-OE-Blast (ERBB2 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-p50(1-435)

    Plasmid

    #44747

    Depositor

    Gourisankar Ghosh

    Article

    Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

    Insert

    p50 (Nfkb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    AA 1-435

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH47732:FCP:NAT

    Plasmid

    #85984

    Purpose

    Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    NAT
    

    Use

    Golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-LYC

    Plasmid

    #53270

    Purpose

    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

    Plasmid

    #160608

    Purpose

    TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    P35s:dCas9:SRDX:tNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ube2v2

    Plasmid

    #31430

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2V2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pEGB 35s:dCas:BRD:tNos (GB1172)

    Plasmid

    #75401

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas9:BRD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-PalmGRET

    Plasmid

    #158221

    Purpose

    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    PalmGFP-Nluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RBM10_pLX307

    Plasmid

    #98371

    Purpose

    Lentiviral expression of RBM10

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    RBM10 (RBM10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Isoform 2

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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