We narrowed to 16,837 results for: por

1. pcDNA3-nuclear-EKAR4

   Plasmid

   #174439

   Purpose

   Nuclear-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   nuclear-EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP-TPD54

   Plasmid

   #172448

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24a-LaM4-EGFP

   Plasmid

   #182641

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, EGFP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQCXIP-cathepsinD-YMH

   Plasmid

   #182644

   Purpose

   Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   cathepsinD
   

   Use

   Retroviral

   Tags

   His6-tag, TS site from proCCK, and myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMRXIB-mRuby3-STX17TM

   Plasmid

   #221744

   Purpose

   Stable expression of mRuby3-STX17TM in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   STX17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-228

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Barcode backbone

   Plasmid

   #158665

   Purpose

   This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   hcRED

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132399

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TB:5xNF- κβ_RE:MiniP

   Plasmid

   #133878

   Purpose

   5 copies of the NF-κβ DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   5 copies of the NF-kb DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dClover2-N1

    Plasmid

    #54538

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

    Depositor

    Michael Davidson

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    dClover2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-mouse vinculin full length (889)

    Plasmid

    #67935

    Purpose

    Mammalian expression of mouse vinculin with GFP fusion

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    Vinculin (Vcl Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L159P in vinculin

    Promoter

    CMV

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-hARFGAP1-HA

    Plasmid

    #206409

    Purpose

    Mammalian expression of hARFGAP1

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

    Insert

    ARFGAP1 (ARFGAP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. V5-LOV-Turbo-NLS

    Plasmid

    #199663

    Purpose

    expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NLS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmGFP-Sec16S

    Plasmid

    #15775

    Depositor

    Benjamin Glick

    Article

    Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

    Insert

    Sec16S (SEC16B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-TPD54

    Plasmid

    #172449

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLR5-CAG-NLS-SNAP

    Plasmid

    #174879

    Purpose

    PiggyBac transposon vector for expression of NLS-SNAP

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    NLS-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge