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pEMS1312

Plasmid

#29151

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Type

Empty backbone

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre

Available Since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1308

Plasmid

#29150

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Type

Empty backbone

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1293

Plasmid

#29144

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre

Available Since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1164

Plasmid

#29079

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1114

Plasmid

#29043

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1488

Plasmid

#29239

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1294

Plasmid

#29145

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-U6-sasgRNACMV- mCherry-WPREpA

Plasmid

#217015

Purpose

S. aureus sgRNAs backbone along with mCherry expression

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-CybGam-WPRE

Plasmid

#207662

Purpose

Control for CybSEP2

Depositor

Sung Han

Article

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

Gamillus

Use

AAV

Mutation

Histidine 86 and 159 to alanines, inserted gamill…

Promoter

hSynapsin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_rpsM_mCerulean3

Plasmid

#124904

Purpose

rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

rpsM promoter and mCerulean3

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTpal-fdc

Plasmid

#78288

Purpose

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

Inserts

PAL2 (PAL2 Mustard Weed)
FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Mutation

*see below

Promoter

trc

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_SRD5A1-mcherry

Plasmid

#159780

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sga-Cas9

Plasmid

#227005

Purpose

Mammalian expression of Nuclease active S. gallolyticus Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKT-IDH1(R132H)-IRES-Katushka

Plasmid

#124257

Purpose

Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)

Expression

Mammalian

Mutation

Arginine 132 is mutated to Histidine. This mutati…

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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THOC4

Plasmid

#156083

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC4 (ALYREF Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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