20,201 results

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha/TRE3G

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-1

Plasmid

#208790

Purpose

Inducibly expresses human Caspase1, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase1 (CASP1 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pOG44_FLP_recombinase (pEHA1338)

Plasmid

#209087

Purpose

FLP recombinase for FRT-mediated integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Insert

FLP recombinase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_P2A_GFP

Plasmid

#112101

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIN-TetR-mCherry

Plasmid

#59417

Purpose

retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repressor protein (TetR)

Use

Retroviral

Tags

mCherry

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS-HA-Bxb1

Plasmid

#51271

Purpose

Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Bxb1

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLSL.R.Ly

Plasmid

#227697

Purpose

Reengineered geminiviral replicon vector

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Article

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN-EphA2-Flag

Plasmid

#102755

Purpose

Expression of EphA2

Depositor

Jin Chen

Article

Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

Insert

EphA2 (EPHA2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET His6 GST TEV LIC cloning vector (1G)

Plasmid

#29655

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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CD40L-SrtA

Plasmid

#121167

Purpose

pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

CD40L-SrtA

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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EBFP2-N1

Plasmid

#54595

Purpose

Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

EBFP2

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3 Control Vector

Plasmid

#212937

Purpose

Control vector with constitutive firefly luciferase expression

Depositor

Debrya Groskreutz

Insert

luc+

Use

Luciferase

Mutation

WT

Promoter

SV40

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LAMP1-mGFP

Plasmid

#34831

Depositor

Esteban Dell'Angelica

Article

Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

Insert

LAMP1 (LAMP1 Human)

Tags

GSTGSTGSTGA linker, Kozak, and mGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pOpen-lambda red operon

Plasmid

#165521

Purpose

Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

lambda red operon

Use

Synthetic Biology

Available Since

Aug. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLS-SV40-mP-EGFP

Plasmid

#137724

Purpose

Lentivirus based EGFP expression vector

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Insert

SV40 enhancer

Use

Lentiviral

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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