We narrowed to 13,580 results for: TIM

1. pSopBC460S

   Plasmid

   #112514

   Purpose

   PsopB driven sopB (catalytically inactive) and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

   Insert

   sopB (catalytically inactive)/sigE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SopB C460S

   Promoter

   PsopB

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-H10-mCherry

   Plasmid

   #66531

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H10

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H10

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-H9-mCherry

   Plasmid

   #66532

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H9

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H9

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiReport PU.1 3' UTR

   Plasmid

   #26530

   Depositor

   David Baltimore

   Article

   O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

   Insert

   PU.1 3' UTR (Spi1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGC4785

   Plasmid

   #113409

   Purpose

   Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

   Depositor

   Guillaume Cambray, Adam Arkin

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Insert

   359_31121113_2
   

   Use

   Synthetic Biology

   Tags

   fused to codon deptimized sfGFP with Flexible lin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-oChD-citrine

   Plasmid

   #50976

   Purpose

   AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   oChD-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   ChD gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_SMFLuc0---MS2

   Plasmid

   #210568

   Purpose

   Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-nonoptimal FLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB_SMFLuc100---MS2

   Plasmid

   #210570

   Purpose

   Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-optimal FLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPO-His-G-Ca-FLITS

   Plasmid

   #191455

   Purpose

   Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   His

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2385-Tier1-PhCMV-fastFT

    Plasmid

    #169518

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2384-Tier1-PhCMV-medFT

    Plasmid

    #169517

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

    Plasmid

    #173462

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

    Plasmid

    #173460

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS41K-mCherry-mNeonGreen (pJJF003)

    Plasmid

    #173459

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

    Plasmid

    #173458

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. L1-neo-TET

    Plasmid

    #51284

    Purpose

    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    L1RE1 (L1RE1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP2-48-merTetO-EFS-BLaR

    Plasmid

    #118712

    Purpose

    Contains an optimized 48-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. eGFP STAT1 S727E

    Plasmid

    #12305

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 S727E (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727E

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1_GEM2-Halo-FRB

    Plasmid

    #197056

    Purpose

    Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eGFP STAT1 S727A

    Plasmid

    #12304

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 S727A (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727A

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBHM2404

    Plasmid

    #173441

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-NAT1
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBHM2406

    Plasmid

    #173442

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-HYGB
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMX-mTAF6

    Plasmid

    #44337

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSetB-KanR-LiLac

    Plasmid

    #184569

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    Tags

    His7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. eGFP STAT1 Y701F

    Plasmid

    #12302

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 Y701F (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Y701F

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pNW-sfGFP(SPS6)

    Plasmid

    #133814

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppta

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 114_UBI_iCre_crystGFP

    Plasmid

    #171797

    Purpose

    Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised iCre under the control of the Ubiquitin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge