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178,767 results

Showing: 6181 - 6200 of 178767 results

  1. pUAS-V5-TurboID-NES

    Plasmid
    #116904
    Purpose
    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.
    Depositor
    Norbert Perrimon
    Publication
    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboID
    Use
    Drosophila expression
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    116904-plasmid-map-sequence-id-224242

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  4. pHAGE-GFP

    Plasmid
    #106281
    Purpose
    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106281-plasmid-map-sequence-id-212556

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  10. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Publication
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    96921-plasmid-map-sequence-id-181715

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  11. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87836-plasmid-map-sequence-id-176310

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  14. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #85006
    Purpose
    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-ires-tTA
    Expression
    Mammalian
    Available since
    Nov. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85006-plasmid-map-sequence-id-167292

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  16. pCDNA3-STAT3-Y705F

    Plasmid
    #74434
    Purpose
    Expression of STAT3-Y705F
    Depositor
    Jie Chen
    Publication
    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Mutations E16K, Y705F
    Promoter
    CMV
    Available since
    May 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74434-plasmid-map-sequence-id-314171

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  20. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71288-plasmid-map-sequence-id-166343

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Showing: 6181 - 6200 of 178767 results