178,767 results
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Plasmid#62541PurposeLTR7-driving reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLTR7-GFP
UseSleeping beauty transposonExpressionMammalianPromoterLTR7Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pET28a-MSP1D1deltaH4H5
Plasmid#71716PurposeMembrane scaffold protein (MSP), helix 4+ helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
ExpressionBacterialAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-6
Plasmid#61566PurposeStandardized mini-Tn5 delivery plasmid, gentamicin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ150
Plasmid#69301PurposeGateway entry vector with pcoCas9 (Plant codon optimized)DepositorInsertpcoCas9 (plant codon-optimized)
UseCRISPRTags3XFLAGMutationNLS fusionAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B
Plasmid#64221PurposeExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherry-P2A-E1BExpressionMammalianPromoterCBh and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Adeno Cas9
Plasmid#64072PurposeAdenoviral vector for the expression of the Cas9DepositorTypeEmpty backboneUseAdenoviralTagsFLAGPromoterU6 and CBhAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ii-Str_ssSBP-EGFP
Plasmid#65277Purposeto synchronize transport of secretory soluble GFP from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
TagsIL-2 signal sequence and Streptavidin Binding Pr…ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CHRNA7
Plasmid#62276Purposemammalian expression of human Alpha7 (CHRNA7)DepositorAvailable sinceMay 21, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
GFP-ASH1L
Plasmid#65369PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertASH1L (ASH1L Human)
TagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHSN6I01
Plasmid#50587PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 that is defective in DNA cleavage; the maiz…Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER13 (miR-LUP)
Plasmid#46936PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Kinesin11-N-18
Plasmid#55067PurposeLocalization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKinesin11 (KIF11 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA
Plasmid#55201PurposePlasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdsRed2
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-14-3-3 ϵ
Plasmid#48797Purposeexpresses human myc-14-3-3ϵ in mammalian cellsDepositorPublicationAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGE31
Plasmid#50929PurposeExpressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRice snoRNA U3 and dual 35S promoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
human Alpha-synuclein
Plasmid#51437Purposehuman codon optimized Alpha-synucleinDepositorInsertAlpha-synuclein (SNCA Human)
UseCloningAvailable sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pHR SFFVp Phy-mCherry-CAAX
Plasmid#50839PurposeOptogenetic PI3K activation system; membrane componentDepositorInsertPhyB 1-908
UseLentiviral and Synthetic BiologyTagsmCherryPromoterSFFVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by